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Applied and Environmental Microbiology, January 2003, p. 679-682, Vol. 69, No. 1
0099-2240/03/$08.00+0 DOI: 10.1128/AEM.69.1.679-682.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.
Max-Planck-Institut für Zellbiologie, Rosenhof, 68526 Ladenburg, Germany,1 Laboratoire de Pathologie Végétale, UMR217 INRA/INA-PG/Université Paris VI, 75231 Paris Cedex 05, France2
Received 3 June 2002/ Accepted 6 October 2002
From necrotic tissue of a Nashi pear tree, 24 Erwinia pyrifoliae strains, found to be identical by pulsed-field gel electrophoresis analysis, were isolated. Thirteen strains were not virulent on immature pears and did not induce a hypersensitive response in tobacco leaves. The defective gene hrpL was complemented with intact genes from E. pyrifoliae and Erwinia amylovora.
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