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Applied and Environmental Microbiology, March 1999, p. 929-935, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Role of the Trichoderma harzianum Endochitinase Gene, ech42, in Mycoparasitism

Carolina Carsolio,1 Nicole Benhamou,2 Shoshan Haran,3 Carlos Cortés,1 Ana Gutiérrez,1 Ilan Chet,3 and Alfredo Herrera-Estrella1,*

Centro de Investigación y Estudios Avanzados, Plant Biotechnology and Genetic Engineering Unit, Irapuato, México1; Université Laval, Ste. Foy, Québec, Canada2; and Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel3

Received 21 May 1998/Accepted 3 December 1998

The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased up to 42-fold in multicopy strains as compared with the wild type, whereas gene disruptants exhibited practically no activity. The densities of chitin labeling of Rhizoctonia solani cell walls, after interactions with gene disruptants were not statistically significantly different than the density of chitin labeling after interactions with the wild type. Finally, no major differences in the efficacies of the strains generated as biocontrol agents against R. solani or Sclerotium rolfsii were observed in greenhouse experiments.

* Corresponding author. Mailing address: Centro de Investigación y Estudios Avanzados, Unidad Irapuato, A.P. 629, 36500 Irapuato, Gto., Mexico. Phone: 52 462 39658. Fax: 52 462 45849. E-mail: aherrera{at}irapuato.ira.cinvestav.mx.

Applied and Environmental Microbiology, March 1999, p. 929-935, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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