A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis

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Applied and Environmental Microbiology, March 1999, p. 1298-1303, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Randomly Amplified Polymorphic DNA Marker Specific for the Bacillus cereus Group Is Diagnostic for Bacillus anthracis

Daniele Daffonchio,¹^,^* Sara Borin,¹ Giuseppe Frova,¹ Romina Gallo,² Elena Mori,² Renato Fani,² and Claudia Sorlini¹

Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, 20133 Milan,¹ and Dipartimento di Biologia Animale e Genetica, Università degli Studi, 50125 Florence,² Italy

Received 22 September 1998/Accepted 3 December 1998

Aiming to develop a DNA marker specific for Bacillus anthracis and able to discriminate this species from Bacillus cereus,Bacillus thuringiensis, and Bacillus mycoides, we applied therandomly amplified polymorphic DNA (RAPD) fingerprinting techniqueto a collection of 101 strains of the genus Bacillus, including61 strains of the B. cereus group. An 838-bp RAPD marker (SG-850)specific for B. cereus, B. thuringiensis, B. anthracis, and B.mycoides was identified. This fragment included a putative (366-nucleotide)open reading frame highly homologous to the ypuA gene of Bacillussubtilis. The restriction analysis of the SG-850 fragment withAluI distinguished B. anthracis from the other species of theB. cereusgroup.

^* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degliStudi di Milano, via Celoria 2, 20133 Milan, Italy. Phone: 39-02-23955845.Fax: 39-02-70630829. E-mail: fonchius{at}imiucca.csi.unimi.it.

Applied and Environmental Microbiology, March 1999, p. 1298-1303, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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