This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Langendijk, P. S.
Right arrow Articles by Welling, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Langendijk, P. S.
Right arrow Articles by Welling, G. W.
Agricola
Right arrow Articles by Langendijk, P. S.
Right arrow Articles by Welling, G. W.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 08 1995, 3069-3075, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples

PS Langendijk, F Schut, GJ Jansen, GC Raangs, GR Kamphuis, MH Wilkinson and GW Welling
Department of Medical Microbiology, University of Groningen, The Netherlands.

Three 16S rRNA hybridization probes were developed and tested for genus- specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination of the genus specificity of the oligonucleotides was performed by whole-cell hybridization with fluorescein isothiocyanate-labelled probes. To this end, cells were fixed on glass slides, hybridized with the probes, and monitored by videomicroscopy. In combination with image analysis, this allowed quantification of the fluorescence per cell and objective evaluation of hybridization experiments. One of the probes developed was used to determine the population of Bifidobacterium spp. in human fecal samples. A comparison was made with results obtained by cultural methods for enumeration. Since both methods gave similar population estimates, it was concluded that all bifidobacteria in feces were culturable. However, since the total culturable counts were only a fraction of the total microscopic counts, the contribution of bifidobacteria to the total intestinal microflora was overestimated by almost 10-fold when cultural methods were used as the sole method for enumeration.


This article has been cited by other articles:

  • Sonoyama, K., Fujiwara, R., Takemura, N., Ogasawara, T., Watanabe, J., Ito, H., Morita, T. (2009). Response of Gut Microbiota to Fasting and Hibernation in Syrian Hamsters. Appl. Environ. Microbiol. 75: 6451-6456 [Abstract] [Full Text]  
  • Stehr, M., Greweling, M. C, Tischer, S., Singh, M., Blocker, H., Monner, D. A, Muller, W. (2009). Charles River altered Schaedler flora (CRASF(R)) remained stable for four years in a mouse colony housed in individually ventilated cages. Lab Anim 43: 362-370 [Abstract] [Full Text]  
  • Vandeplas, S., Dauphin, R. D., Thiry, C., Beckers, Y., Welling, G. W., Thonart, P., Thewis, A. (2009). Efficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium. Poult. Sci. 88: 1643-1654 [Abstract] [Full Text]  
  • Di Cagno, R., Rizzello, C. G., Gagliardi, F., Ricciuti, P., Ndagijimana, M., Francavilla, R., Guerzoni, M. E., Crecchio, C., Gobbetti, M., De Angelis, M. (2009). Different Fecal Microbiotas and Volatile Organic Compounds in Treated and Untreated Children with Celiac Disease. Appl. Environ. Microbiol. 75: 3963-3971 [Abstract] [Full Text]  
  • Paliy, O., Kenche, H., Abernathy, F., Michail, S. (2009). High-Throughput Quantitative Analysis of the Human Intestinal Microbiota with a Phylogenetic Microarray. Appl. Environ. Microbiol. 75: 3572-3579 [Abstract] [Full Text]  
  • Hong, P.-Y., Yap, G. C., Lee, B. W., Chua, K. Y., Liu, W.-T. (2009). Hierarchical Oligonucleotide Primer Extension as a Time- and Cost-Effective Approach for Quantitative Determination of Bifidobacterium spp. in Infant Feces. Appl. Environ. Microbiol. 75: 2573-2576 [Abstract] [Full Text]  
  • Ruiz, L., Coute, Y., Sanchez, B., de los Reyes-Gavilan, C. G., Sanchez, J.-C., Margolles, A. (2009). The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment. Microbiology 155: 957-967 [Abstract] [Full Text]  
  • Nakamura, N., Gaskins, H. R., Collier, C. T., Nava, G. M., Rai, D., Petschow, B., Russell, W. M., Harris, C., Mackie, R. I., Wampler, J. L., Walker, D. C. (2009). Molecular Ecological Analysis of Fecal Bacterial Populations from Term Infants Fed Formula Supplemented with Selected Blends of Prebiotics. Appl. Environ. Microbiol. 75: 1121-1128 [Abstract] [Full Text]  
  • Whelan, K., Judd, P. A, Tuohy, K. M, Gibson, G. R, Preedy, V. R, Taylor, M. A (2009). Fecal microbiota in patients receiving enteral feeding are highly variable and may be altered in those who develop diarrhea. Am. J. Clin. Nutr. 89: 240-247 [Abstract] [Full Text]  
  • Vulevic, J., Drakoularakou, A., Yaqoob, P., Tzortzis, G., Gibson, G. R (2008). Modulation of the fecal microflora profile and immune function by a novel trans-galactooligosaccharide mixture (B-GOS) in healthy elderly volunteers. Am. J. Clin. Nutr. 88: 1438-1446 [Abstract] [Full Text]  
  • Gao, Z., Li, B., Zheng, C., Wang, G. (2008). Molecular Detection of Fungal Communities in the Hawaiian Marine Sponges Suberites zeteki and Mycale armata. Appl. Environ. Microbiol. 74: 6091-6101 [Abstract] [Full Text]  
  • Siggers, R. H., Siggers, J., Boye, M., Thymann, T., Molbak, L., Leser, T., Jensen, B. B., Sangild, P. T. (2008). Early Administration of Probiotics Alters Bacterial Colonization and Limits Diet-Induced Gut Dysfunction and Severity of Necrotizing Enterocolitis in Preterm Pigs. J. Nutr. 138: 1437-1444 [Abstract] [Full Text]  
  • Mandalari, G., Nueno-Palop, C., Bisignano, G., Wickham, M. S. J., Narbad, A. (2008). Potential Prebiotic Properties of Almond (Amygdalus communis L.) Seeds. Appl. Environ. Microbiol. 74: 4264-4270 [Abstract] [Full Text]  
  • Scholtens, P. A. M. J., Alliet, P., Raes, M., Alles, M. S., Kroes, H., Boehm, G., Knippels, L. M. J., Knol, J., Vandenplas, Y. (2008). Fecal Secretory Immunoglobulin A Is Increased in Healthy Infants Who Receive a Formula with Short-Chain Galacto-Oligosaccharides and Long-Chain Fructo-Oligosaccharides. J. Nutr. 138: 1141-1147 [Abstract] [Full Text]  
  • Depeint, F., Tzortzis, G., Vulevic, J., I'Anson, K., Gibson, G. R (2008). Prebiotic evaluation of a novel galactooligosaccharide mixture produced by the enzymatic activity of Bifidobacterium bifidum NCIMB 41171, in healthy humans: a randomized, double-blind, crossover, placebo-controlled intervention study. Am. J. Clin. Nutr. 87: 785-791 [Abstract] [Full Text]  
  • Lamendella, R., Domingo, J. W. S., Kelty, C., Oerther, D. B. (2008). Bifidobacteria in Feces and Environmental Waters. Appl. Environ. Microbiol. 74: 575-584 [Abstract] [Full Text]  
  • Gori, A., Tincati, C., Rizzardini, G., Torti, C., Quirino, T., Haarman, M., Ben Amor, K., van Schaik, J., Vriesema, A., Knol, J., Marchetti, G., Welling, G., Clerici, M. (2008). Early Impairment of Gut Function and Gut Flora Supporting a Role for Alteration of Gastrointestinal Mucosa in Human Immunodeficiency Virus Pathogenesis. J. Clin. Microbiol. 46: 757-758 [Abstract] [Full Text]  
  • Nadal, I., Donant, E., Ribes-Koninckx, C., Calabuig, M., Sanz, Y. (2007). Imbalance in the composition of the duodenal microbiota of children with coeliac disease. J Med Microbiol 56: 1669-1674 [Abstract] [Full Text]  
  • Ahmed, S., Macfarlane, G. T., Fite, A., McBain, A. J., Gilbert, P., Macfarlane, S. (2007). Mucosa-Associated Bacterial Diversity in Relation to Human Terminal Ileum and Colonic Biopsy Samples. Appl. Environ. Microbiol. 73: 7435-7442 [Abstract] [Full Text]  
  • Shadid, R., Haarman, M., Knol, J., Theis, W., Beermann, C., Rjosk-Dendorfer, D., Schendel, D. J, Koletzko, B. V, Krauss-Etschmann, S. (2007). Effects of galactooligosaccharide and long-chain fructooligosaccharide supplementation during pregnancy on maternal and neonatal microbiota and immunity a randomized, double-blind, placebo-controlled study. Am. J. Clin. Nutr. 86: 1426-1437 [Abstract] [Full Text]  
  • Belenguer, A., Duncan, S. H., Holtrop, G., Anderson, S. E., Lobley, G. E., Flint, H. J. (2007). Impact of pH on Lactate Formation and Utilization by Human Fecal Microbial Communities. Appl. Environ. Microbiol. 73: 6526-6533 [Abstract] [Full Text]  
  • Zhou, Z., Pons, M. N., Raskin, L., Zilles, J. L. (2007). Automated Image Analysis for Quantitative Fluorescence In Situ Hybridization with Environmental Samples. Appl. Environ. Microbiol. 73: 2956-2962 [Abstract] [Full Text]  
  • Carlier, J.-P., K'ouas, G., Han, X. Y. (2007). Moryella indoligenes gen. nov., sp. nov., an anaerobic bacterium isolated from clinical specimens. Int. J. Syst. Evol. Microbiol. 57: 725-729 [Abstract] [Full Text]  
  • Schoniger-Hekele, M., Petermann, D., Weber, B., Muller, C. (2007). Tropheryma whipplei in the Environment: Survey of Sewage Plant Influxes and Sewage Plant Workers. Appl. Environ. Microbiol. 73: 2033-2035 [Abstract] [Full Text]  
  • Lunn, J.C., Kuhnle, G., Mai, V., Frankenfeld, C., Shuker, D.E.G., Glen, R. C., Goodman, J.M., Pollock, J.R.A., Bingham, S.A. (2007). The effect of haem in red and processed meat on the endogenous formation of N-nitroso compounds in the upper gastrointestinal tract. Carcinogenesis 28: 685-690 [Abstract] [Full Text]  
  • Ben Amor, K., Vaughan, E. E., de Vos, W. M. (2007). Advanced Molecular Tools for the Identification of Lactic Acid Bacteria. J. Nutr. 137: 741S-747S [Abstract] [Full Text]  
  • Roberfroid, M. (2007). Prebiotics: The Concept Revisited. J. Nutr. 137: 830S-837S [Abstract] [Full Text]  
  • Swidsinski, A, Goktas, O, Bessler, C, Loening-Baucke, V, Hale, L P, Andree, H, Weizenegger, M, Holzl, M, Scherer, H, Lochs, H (2007). Spatial organisation of microbiota in quiescent adenoiditis and tonsillitis. J. Clin. Pathol. 60: 253-260 [Abstract] [Full Text]  
  • Duncan, S. H., Belenguer, A., Holtrop, G., Johnstone, A. M., Flint, H. J., Lobley, G. E. (2007). Reduced Dietary Intake of Carbohydrates by Obese Subjects Results in Decreased Concentrations of Butyrate and Butyrate-Producing Bacteria in Feces. Appl. Environ. Microbiol. 73: 1073-1078 [Abstract] [Full Text]  
  • Dinoto, A., Marques, T. M., Sakamoto, K., Fukiya, S., Watanabe, J., Ito, S., Yokota, A. (2006). Population Dynamics of Bifidobacterium Species in Human Feces during Raffinose Administration Monitored by Fluorescence In Situ Hybridization-Flow Cytometry. Appl. Environ. Microbiol. 72: 7739-7747 [Abstract] [Full Text]  
  • Moroni, O., Kheadr, E., Boutin, Y., Lacroix, C., Fliss, I. (2006). Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin. Appl. Environ. Microbiol. 72: 6894-6901 [Abstract] [Full Text]  
  • Mohan, R., Koebnick, C., Schildt, J., Schmidt, S., Mueller, M., Possner, M., Radke, M., Blaut, M. (2006). Effects of Bifidobacterium lactis Bb12 Supplementation on Intestinal Microbiota of Preterm Infants: a Double-Blind, Placebo-Controlled, Randomized Study. J. Clin. Microbiol. 44: 4025-4031 [Abstract] [Full Text]  
  • Vanhoutte, T., De Preter, V., De Brandt, E., Verbeke, K., Swings, J., Huys, G. (2006). Molecular Monitoring of the Fecal Microbiota of Healthy Human Subjects during Administration of Lactulose and Saccharomyces boulardii. Appl. Environ. Microbiol. 72: 5990-5997 [Abstract] [Full Text]  
  • Macfarlane, S., Macfarlane, G. T. (2006). Composition and Metabolic Activities of Bacterial Biofilms Colonizing Food Residues in the Human Gut. Appl. Environ. Microbiol. 72: 6204-6211 [Abstract] [Full Text]  
  • Van der Meulen, R., Adriany, T., Verbrugghe, K., De Vuyst, L. (2006). Kinetic Analysis of Bifidobacterial Metabolism Reveals a Minor Role for Succinic Acid in the Regeneration of NAD+ through Its Growth-Associated Production. Appl. Environ. Microbiol. 72: 5204-5210 [Abstract] [Full Text]  
  • Friedrich, U., Lenke, J. (2006). Improved enumeration of lactic Acid bacteria in mesophilic dairy starter cultures by using multiplex quantitative real-time PCR and flow cytometry-fluorescence in situ hybridization.. Appl. Environ. Microbiol. 72: 4163-4171 [Abstract] [Full Text]  
  • Kuhbacher, T, Ott, S J, Helwig, U, Mimura, T, Rizzello, F, Kleessen, B, Gionchetti, P, Blaut, M, Campieri, M, Folsch, U R, Kamm, M A, Schreiber, S (2006). Bacterial and fungal microbiota in relation to probiotic therapy (VSL#3) in pouchitis. Gut 55: 833-841 [Abstract] [Full Text]  
  • Lindsay, J O, Whelan, K, Stagg, A J, Gobin, P, Al-Hassi, H O, Rayment, N, Kamm, M A, Knight, S C, Forbes, A (2006). Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease. Gut 55: 348-355 [Abstract] [Full Text]  
  • Weng, L., Rubin, E. M., Bristow, J. (2006). Application of sequence-based methods in human microbial ecology. Genome Res 16: 316-322 [Abstract] [Full Text]  
  • Van der Meulen, R., Makras, L., Verbrugghe, K., Adriany, T., De Vuyst, L. (2006). In Vitro Kinetic Analysis of Oligofructose Consumption by Bacteroides and Bifidobacterium spp. Indicates Different Degradation Mechanisms. Appl. Environ. Microbiol. 72: 1006-1012 [Abstract] [Full Text]  
  • Mueller, S., Saunier, K., Hanisch, C., Norin, E., Alm, L., Midtvedt, T., Cresci, A., Silvi, S., Orpianesi, C., Verdenelli, M. C., Clavel, T., Koebnick, C., Zunft, H.-J. F., Dore, J., Blaut, M. (2006). Differences in Fecal Microbiota in Different European Study Populations in Relation to Age, Gender, and Country: a Cross-Sectional Study. Appl. Environ. Microbiol. 72: 1027-1033 [Abstract] [Full Text]  
  • Dinoto, A., Suksomcheep, A., Ishizuka, S., Kimura, H., Hanada, S., Kamagata, Y., Asano, K., Tomita, F., Yokota, A. (2006). Modulation of Rat Cecal Microbiota by Administration of Raffinose and Encapsulated Bifidobacterium breve. Appl. Environ. Microbiol. 72: 784-792 [Abstract] [Full Text]  
  • Scupham, A. J, Presley, L. L., Wei, B., Bent, E., Griffith, N., McPherson, M., Zhu, F., Oluwadara, O., Rao, N., Braun, J., Borneman, J. (2006). Abundant and Diverse Fungal Microbiota in the Murine Intestine. Appl. Environ. Microbiol. 72: 793-801 [Abstract] [Full Text]  
  • Manderson, K., Pinart, M., Tuohy, K. M., Grace, W. E., Hotchkiss, A. T., Widmer, W., Yadhav, M. P., Gibson, G. R., Rastall, R. A. (2005). In Vitro Determination of Prebiotic Properties of Oligosaccharides Derived from an Orange Juice Manufacturing By-Product Stream. Appl. Environ. Microbiol. 71: 8383-8389 [Abstract] [Full Text]  
  • Clavel, T., Fallani, M., Lepage, P., Levenez, F., Mathey, J., Rochet, V., Serezat, M., Sutren, M., Henderson, G., Bennetau-Pelissero, C., Tondu, F., Blaut, M., Dore, J., Coxam, V. (2005). Isoflavones and Functional Foods Alter the Dominant Intestinal Microbiota in Postmenopausal Women. J. Nutr. 135: 2786-2792 [Abstract] [Full Text]  
  • Ruas-Madiedo, P., Hernandez-Barranco, A., Margolles, A., de los Reyes-Gavilan, C. G. (2005). A Bile Salt-Resistant Derivative of Bifidobacterium animalis Has an Altered Fermentation Pattern When Grown on Glucose and Maltose. Appl. Environ. Microbiol. 71: 6564-6570 [Abstract] [Full Text]  
  • Macfarlane, S., Woodmansey, E. J., Macfarlane, G. T. (2005). Colonization of Mucin by Human Intestinal Bacteria and Establishment of Biofilm Communities in a Two-Stage Continuous Culture System. Appl. Environ. Microbiol. 71: 7483-7492 [Abstract] [Full Text]  
  • Clavel, T., Henderson, G., Alpert, C.-A., Philippe, C., Rigottier-Gois, L., Dore, J., Blaut, M. (2005). Intestinal Bacterial Communities That Produce Active Estrogen-Like Compounds Enterodiol and Enterolactone in Humans. Appl. Environ. Microbiol. 71: 6077-6085 [Abstract] [Full Text]  
  • van Tongeren, S. P., Slaets, J. P. J., Harmsen, H. J. M., Welling, G. W. (2005). Fecal Microbiota Composition and Frailty. Appl. Environ. Microbiol. 71: 6438-6442 [Abstract] [Full Text]  
  • Parracho, H. M., Bingham, M. O, Gibson, G. R, McCartney, A. L (2005). Differences between the gut microflora of children with autistic spectrum disorders and that of healthy children. J Med Microbiol 54: 987-991 [Abstract] [Full Text]  
  • Ben-Amor, K., Heilig, H., Smidt, H., Vaughan, E. E., Abee, T., de Vos, W. M. (2005). Genetic Diversity of Viable, Injured, and Dead Fecal Bacteria Assessed by Fluorescence-Activated Cell Sorting and 16S rRNA Gene Analysis. Appl. Environ. Microbiol. 71: 4679-4689 [Abstract] [Full Text]  
  • Mennink-Kersten, M. A. S. H., Ruegebrink, D., Klont, R. R., Warris, A., Gavini, F., Op den Camp, H. J. M., Verweij, P. E. (2005). Bifidobacterial Lipoglycan as a New Cause for False-Positive Platelia Aspergillus Enzyme-Linked Immunosorbent Assay Reactivity. J. Clin. Microbiol. 43: 3925-3931 [Abstract] [Full Text]  
  • Collado, M. C., Moreno, Y., Hernandez, E., Cobo, J. M., Hernandez, M. (2005). Note. In Vitro Viability of Bifidobacterium Strains Isolated from Commercial Dairy Products Exposed to Human Gastrointestinal Conditions. Food Science and Technology International 11: 307-314 [Abstract]  
  • Whelan, K., Judd, P. A., Preedy, V. R., Simmering, R., Jann, A., Taylor, M. A. (2005). Fructooligosaccharides and Fiber Partially Prevent the Alterations in Fecal Microbiota and Short-Chain Fatty Acid Concentrations Caused by Standard Enteral Formula in Healthy Humans. J. Nutr. 135: 1896-1902 [Abstract] [Full Text]  
  • Walker, A. W., Duncan, S. H., McWilliam Leitch, E. C., Child, M. W., Flint, H. J. (2005). pH and Peptide Supply Can Radically Alter Bacterial Populations and Short-Chain Fatty Acid Ratios within Microbial Communities from the Human Colon. Appl. Environ. Microbiol. 71: 3692-3700 [Abstract] [Full Text]  
  • Swidsinski, A., Weber, J., Loening-Baucke, V., Hale, L. P., Lochs, H. (2005). Spatial Organization and Composition of the Mucosal Flora in Patients with Inflammatory Bowel Disease. J. Clin. Microbiol. 43: 3380-3389 [Abstract] [Full Text]  
  • Tzortzis, G., Goulas, A. K., Gee, J. M., Gibson, G. R. (2005). A Novel Galactooligosaccharide Mixture Increases the Bifidobacterial Population Numbers in a Continuous In Vitro Fermentation System and in the Proximal Colonic Contents of Pigs In Vivo. J. Nutr. 135: 1726-1731 [Abstract] [Full Text]  
  • Haarman, M., Knol, J. (2005). Quantitative Real-Time PCR Assays To Identify and Quantify Fecal Bifidobacterium Species in Infants Receiving a Prebiotic Infant Formula. Appl. Environ. Microbiol. 71: 2318-2324 [Abstract] [Full Text]  
  • Lahtinen, S. J., Gueimonde, M., Ouwehand, A. C., Reinikainen, J. P., Salminen, S. J. (2005). Probiotic Bacteria May Become Dormant during Storage. Appl. Environ. Microbiol. 71: 1662-1663 [Abstract] [Full Text]  
  • Swidsinski, A, Schlien, P, Pernthaler, A, Gottschalk, U, Barlehner, E, Decker, G, Swidsinski, S, Strassburg, J, Loening-Baucke, V, Hoffmann, U, Seehofer, D, Hale, L P, Lochs, H (2005). Bacterial biofilm within diseased pancreatic and biliary tracts. Gut 54: 388-395 [Abstract] [Full Text]  
  • Smirnov, A., Perez, R., Amit-Romach, E., Sklan, D., Uni, Z. (2005). Mucin Dynamics and Microbial Populations in Chicken Small Intestine Are Changed by Dietary Probiotic and Antibiotic Growth Promoter Supplementation. J. Nutr. 135: 187-192 [Abstract] [Full Text]  
  • Jernberg, C., Sullivan, A., Edlund, C., Jansson, J. K. (2005). Monitoring of Antibiotic-Induced Alterations in the Human Intestinal Microflora and Detection of Probiotic Strains by Use of Terminal Restriction Fragment Length Polymorphism. Appl. Environ. Microbiol. 71: 501-506 [Abstract] [Full Text]  
  • Matsuki, T., Watanabe, K., Fujimoto, J., Takada, T., Tanaka, R. (2004). Use of 16S rRNA Gene-Targeted Group-Specific Primers for Real-Time PCR Analysis of Predominant Bacteria in Human Feces. Appl. Environ. Microbiol. 70: 7220-7228 [Abstract] [Full Text]  
  • Song, Y., Liu, C., Finegold, S. M. (2004). Real-Time PCR Quantitation of Clostridia in Feces of Autistic Children. Appl. Environ. Microbiol. 70: 6459-6465 [Abstract] [Full Text]  
  • Langlands, S J, Hopkins, M J, Coleman, N, Cummings, J H (2004). Prebiotic carbohydrates modify the mucosa associated microflora of the human large bowel. Gut 53: 1610-1616 [Abstract] [Full Text]  
  • Suchodolski, J. S., Ruaux, C. G., Steiner, J. M., Fetz, K., Williams, D. A. (2004). Application of Molecular Fingerprinting for Qualitative Assessment of Small-Intestinal Bacterial Diversity in Dogs. J. Clin. Microbiol. 42: 4702-4708 [Abstract] [Full Text]  
  • Vulevic, J., McCartney, A. L., Gee, J. M., Johnson, I. T., Gibson, G. R. (2004). Microbial Species Involved in Production of 1,2-sn-Diacylglycerol and Effects of Phosphatidylcholine on Human Fecal Microbiota. Appl. Environ. Microbiol. 70: 5659-5666 [Abstract] [Full Text]  
  • Probert, H. M., Apajalahti, J. H. A., Rautonen, N., Stowell, J., Gibson, G. R. (2004). Polydextrose, Lactitol, and Fructo-Oligosaccharide Fermentation by Colonic Bacteria in a Three-Stage Continuous Culture System. Appl. Environ. Microbiol. 70: 4505-4511 [Abstract] [Full Text]  
  • Rastall, R. A. (2004). Bacteria in the Gut: Friends and Foes and How to Alter the Balance. J. Nutr. 134: 2022S-2026S [Abstract] [Full Text]  
  • Gueimonde, M., Tolkko, S., Korpimaki, T., Salminen, S. (2004). New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples. Appl. Environ. Microbiol. 70: 4165-4169 [Abstract] [Full Text]  
  • Bartosch, S., Fite, A., Macfarlane, G. T., McMurdo, M. E. T. (2004). Characterization of Bacterial Communities in Feces from Healthy Elderly Volunteers and Hospitalized Elderly Patients by Using Real-Time PCR and Effects of Antibiotic Treatment on the Fecal Microbiota. Appl. Environ. Microbiol. 70: 3575-3581 [Abstract] [Full Text]  
  • Ott, S. J., Musfeldt, M., Ullmann, U., Hampe, J., Schreiber, S. (2004). Quantification of Intestinal Bacterial Populations by Real-Time PCR with a Universal Primer Set and Minor Groove Binder Probes: a Global Approach to the Enteric Flora. J. Clin. Microbiol. 42: 2566-2572 [Abstract] [Full Text]  
  • Sarma-Rupavtarm, R. B., Ge, Z., Schauer, D. B., Fox, J. G., Polz, M. F. (2004). Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract. Appl. Environ. Microbiol. 70: 2791-2800 [Abstract] [Full Text]  
  • Ott, S J, Musfeldt, M, Wenderoth, D F, Hampe, J, Brant, O, Folsch, U R, Timmis, K N, Schreiber, S (2004). Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. Gut 53: 685-693 [Abstract] [Full Text]  
  • Fite, A, Macfarlane, G T, Cummings, J H, Hopkins, M J, Kong, S C, Furrie, E, Macfarlane, S (2004). Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction. Gut 53: 523-529 [Abstract] [Full Text]  
  • Simpson, P. J., Ross, R. P., Fitzgerald, G. F., Stanton, C. (2004). Bifidobacterium psychraerophilum sp. nov. and Aeriscardovia aeriphila gen. nov., sp. nov., isolated from a porcine caecum. Int. J. Syst. Evol. Microbiol. 54: 401-406 [Abstract] [Full Text]  
  • Mai, V., Katki, H. A., Harmsen, H., Gallaher, D., Schatzkin, A., Baer, D. J., Clevidence, B. (2004). Effects of a Controlled Diet and Black Tea Drinking on the Fecal Microflora Composition and the Fecal Bile Acid Profile of Human Volunteers in a Double-Blinded Randomized Feeding Study. J. Nutr. 134: 473-478 [Abstract] [Full Text]  
  • Matsuki, T., Watanabe, K., Fujimoto, J., Kado, Y., Takada, T., Matsumoto, K., Tanaka, R. (2004). Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria. Appl. Environ. Microbiol. 70: 167-173 [Abstract] [Full Text]  
  • Nielsen, D. S., Moller, P. L., Rosenfeldt, V., Paerregaard, A., Michaelsen, K. F., Jakobsen, M. (2003). Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, and Other Lactic Acid Bacteria in the Human Colon. Appl. Environ. Microbiol. 69: 7545-7548 [Abstract] [Full Text]  
  • Hopkins, M. J., Englyst, H. N., Macfarlane, S., Furrie, E., Macfarlane, G. T., McBain, A. J. (2003). Degradation of Cross-Linked and Non-Cross-Linked Arabinoxylans by the Intestinal Microbiota in Children. Appl. Environ. Microbiol. 69: 6354-6360 [Abstract] [Full Text]  
  • Apajalahti, J. H. A., Kettunen, A., Nurminen, P. H., Jatila, H., Holben, W. E. (2003). Selective Plating Underestimates Abundance and Shows Differential Recovery of Bifidobacterial Species from Human Feces. Appl. Environ. Microbiol. 69: 5731-5735 [Abstract] [Full Text]  
  • Zabarovsky, E. R., Petrenko, L., Protopopov, A., Vorontsova, O., Kutsenko, A. S., Zhao, Y., Kilosanidze, G., Zabarovska, V., Rakhmanaliev, E., Pettersson, B., Kashuba, V. I., Ljungqvist, O., Norin, E., Midtvedt, T., Mollby, R., Winberg, G., Ernberg, I. (2003). Restriction site tagged (RST) microarrays: a novel technique to study the species composition of complex microbial systems. Nucleic Acids Res 31: e95-e95 [Abstract] [Full Text]  
  • Ventura, M., Meylan, V., Zink, R. (2003). Identification and Tracing of Bifidobacterium Species by Use of Enterobacterial Repetitive Intergenic Consensus Sequences. Appl. Environ. Microbiol. 69: 4296-4301 [Abstract] [Full Text]  
  • Nebra, Y., Bonjoch, X., Blanch, A. R. (2003). Use of Bifidobacterium dentium as an Indicator of the Origin of Fecal Water Pollution. Appl. Environ. Microbiol. 69: 2651-2656 [Abstract] [Full Text]  
  • Duncan, S. H., Scott, K. P., Ramsay, A. G., Harmsen, H. J. M., Welling, G. W., Stewart, C. S., Flint, H. J. (2003). Effects of Alternative Dietary Substrates on Competition between Human Colonic Bacteria in an Anaerobic Fermentor System. Appl. Environ. Microbiol. 69: 1136-1142 [Abstract] [Full Text]  
  • Nagashima, K., Hisada, T., Sato, M., Mochizuki, J. (2003). Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces. Appl. Environ. Microbiol. 69: 1251-1262 [Abstract] [Full Text]  
  • Zabarovska, V., Kutsenko, A. S., Petrenko, L., Kilosanidze, G., Ljungqvist, O., Norin, E., Midtvedt, T., Winberg, G., Mollby, R., Kashuba, V. I., Ernberg, I., Zabarovsky, E. R. (2003). NotI passporting to identify species composition of complex microbial systems. Nucleic Acids Res 31: e5-e5 [Abstract] [Full Text]  
  • Mikkelsen, L. L., Bendixen, C., Jakobsen, M., Jensen, B. B. (2003). Enumeration of Bifidobacteria in Gastrointestinal Samples from Piglets. Appl. Environ. Microbiol. 69: 654-658 [Abstract] [Full Text]  
  • Zoetendal, E. G., Plugge, C. M., Akkermans, A. D. L., de Vos, W. M. (2003). Victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces. Int. J. Syst. Evol. Microbiol. 53: 211-215 [Abstract] [Full Text]  
  • Malinen, E., Kassinen, A., Rinttila, T., Palva, A. (2003). Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology 149: 269-277 [Abstract] [Full Text]  
  • Huijsdens, X. W., Linskens, R. K., Mak, M., Meuwissen, S. G. M., Vandenbroucke-Grauls, C. M. J. E., Savelkoul, P. H. M. (2002). Quantification of Bacteria Adherent to Gastrointestinal Mucosa by Real-Time PCR. J. Clin. Microbiol. 40: 4423-4427 [Abstract] [Full Text]  
  • Matsuki, T., Watanabe, K., Fujimoto, J., Miyamoto, Y., Takada, T., Matsumoto, K., Oyaizu, H., Tanaka, R. (2002). Development of 16S rRNA-Gene-Targeted Group-Specific Primers for the Detection and Identification of Predominant Bacteria in Human Feces. Appl. Environ. Microbiol. 68: 5445-5451 [Abstract] [Full Text]  
  • Salzman, N. H., de Jong, H., Paterson, Y., Harmsen, H. J. M., Welling, G. W., Bos, N. A. (2002). Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148: 3651-3660 [Abstract] [Full Text]  
  • Kirjavainen, P V, Arvola, T, Salminen, S J, Isolauri, E (2002). Aberrant composition of gut microbiota of allergic infants: a target of bifidobacterial therapy at weaning?. Gut 51: 51-55 [Abstract] [Full Text]  
  • Harmsen, H. J. M., Raangs, G. C., He, T., Degener, J. E., Welling, G. W. (2002). Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human Feces. Appl. Environ. Microbiol. 68: 2982-2990 [Abstract] [Full Text]  
  • Requena, T., Burton, J., Matsuki, T., Munro, K., Simon, M. A., Tanaka, R., Watanabe, K., Tannock, G. W. (2002). Identification, Detection, and Enumeration of Human Bifidobacterium Species by PCR Targeting the Transaldolase Gene. Appl. Environ. Microbiol. 68: 2420-2427 [Abstract] [Full Text]  
  • Heilig, H. G.H.J., Zoetendal, E. G., Vaughan, E. E., Marteau, P., Akkermans, A. D.L., de Vos, W. M. (2002). Molecular Diversity of Lactobacillus spp. and Other Lactic Acid Bacteria in the Human Intestine as Determined by Specific Amplification of 16S Ribosomal DNA. Appl. Environ. Microbiol. 68: 114-123 [Abstract] [Full Text]  
  • Ventura, M., Reniero, R., Zink, R. (2001). Specific Identification and Targeted Characterization of Bifidobacterium lactis from Different Environmental Isolates by a Combined Multiplex-PCR Approach. Appl. Environ. Microbiol. 67: 2760-2765 [Abstract] [Full Text]  
  • McCracken, V. J., Simpson, J. M., Mackie, R. I., Gaskins, H. R. (2001). Molecular Ecological Analysis of Dietary and Antibiotic-Induced Alterations of the Mouse Intestinal Microbiota. J. Nutr. 131: 1862-1870 [Abstract] [Full Text]  
  • Satokari, R. M., Vaughan, E. E., Akkermans, A. D. L., Saarela, M., de Vos, W. M. (2001). Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol. 67: 504-513 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»