AEM Accepts, published online ahead of print on 30 October 2009

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Appl. Environ. Microbiol. doi:10.1128/AEM.01745-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Aromatic hydroxylation of indan by the o-xylene-degrading Rhodococcus sp. strain DK17

Dockyu Kim, Choong Hwan Lee, Jung Nam Choi, Ki Young Choi, Gerben J. Zylstra, and Eungbin Kim^*

Polar BioCenter, Korea Polar Research Institute, KORDI, Incheon 406-840, Korea; Division of Life Bioscience and Biotechnology, IBST, Konkuk University, Seoul 143-713, Korea; Department of Biology, Yonsei University, Seoul 120-749, Korea; Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Jersey 08901-8520, USA

^* To whom correspondence should be addressed. Email: eungbin{at}yonsei.ac.kr.

The metabolically versatile Rhodococcus sp. strain DK17 utilizes indan as a growth substrate via the o-xylene pathway. Metabolite and RT-PCR analyses indicate that o-xylene dioxygenase hydroxylates indan at the 4,5 position of the aromatic moiety to form cis-indan-4,5-dihydrodiol, which is dehydrogenated to 4,5-indandiol by a dehydrogenase. 4,5-indandiol undergoes ring cleavage by a meta-cleavage dioxygenase.