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Appl. Environ. Microbiol. doi:10.1128/AEM.01527-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Isolation of Rhodococcus sp. ECU0066: A New Sulfide Monooxygenase Producing Strain for Asymmetric Sulfoxidation

Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China

^* To whom correspondence should be addressed. Email: jianhexu{at}ecust.edu.cn.

	Abstract

A new and efficient sulfide monooxygenase-producing strain, no. ECU0066, was isolated and identified as Rhodococcus sp., which could transform phenyl methyl sulfide (PMS) to (S)-sulfoxide with 99% ee (enantiomeric excess) via two steps of enantioselective oxidations. Its enzyme activity could be effectively induced by adding PMS or phenyl methyl sulfoxide (PMSO) directly to a rich medium at the early log phase (6 h) of fermentation, resulting in over 10 times higher production of the enzyme. This bacterial strain also displayed fairly good activity and enantioselectivity towards seven other sulfides, indicating a good potential for practical application in asymmetric synthesis of chiral sulfoxides.