AEM Accepts, published online ahead of print on 16 October 2009
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Appl. Environ. Microbiol. doi:10.1128/AEM.01203-09
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Optimisation of a rapid viability assay for Mycobacterium avium paratuberculosis (MAP) using alamarBlue

James Carroll, Pierre Douarre, Aidan Coffey, Jim Buckley, Bill Cashman, Kevin O'Farrell, and Jim O'Mahony*

Department of Biological Sciences, Cork Institute of Technology, Cork; Veterinary Department, Cork County Council, County Hall, Cork, Ireland

* To whom correspondence should be addressed. Email: jim.omahony{at}cit.


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Abstract

A microtitre alamar blue assay (MABA) was adapted and optimised for Mycobacterium avium paratuberculosis. Using cell concentrations ranging from 104 – 108 CFU/ml, a minimum incubation time to indicate viability was obtained after 24 hours. Rifampicin was used to demonstrate that this method has applications for high-throughput screening against MAP.






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