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Applied and Environmental Microbiology, August 2008, p. 5146-5152, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00944-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Group 2 Sigma Factor SigB of Corynebacterium glutamicum Positively Regulates Glucose Metabolism under Conditions of Oxygen Deprivation{triangledown} ,{dagger}

Shigeki Ehira, Tomokazu Shirai, Haruhiko Teramoto, Masayuki Inui, and Hideaki Yukawa*

Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan

Received 25 April 2008/ Accepted 16 June 2008

The sigB gene of Corynebacterium glutamicum encodes a group 2 sigma factor of RNA polymerase. Under conditions of oxygen deprivation, the sigB gene is upregulated and cells exhibit high productivity of organic acids as a result of an elevated glucose consumption rate. Using DNA microarray and quantitative reverse transcription-PCR (RT-PCR) analyses, we found that sigB disruption led to reduced transcript levels of genes involved in the metabolism of glucose into organic acids. This in turn resulted in retardation of glucose consumption by cells under conditions of oxygen deprivation. These results indicate that SigB is involved in positive regulation of glucose metabolism genes and enhances glucose consumption under conditions of oxygen deprivation. Moreover, sigB disruption reduced the transcript levels of genes involved in various cellular functions, including the glucose metabolism genes not only in the growth-arrested cells under conditions of oxygen deprivation but also in the cells during aerobic exponential growth, suggesting that SigB functions as another vegetative sigma factor.

* Corresponding author. Mailing address: Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto 619-0292, Japan. Phone: 81-774-75-2308. Fax: 81-774-75-2321. E-mail: mmg-lab{at}rite.or.jp

{triangledown} Published ahead of print on 20 June 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, August 2008, p. 5146-5152, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00944-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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