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Applied and Environmental Microbiology, August 2008, p. 5106-5112, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00253-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Purification, Characterization, and Gene Cloning of Ceriporiopsis sp. Strain MD-1 Peroxidases That Decolorize Human Hair Melanin{triangledown}

Kenji Nagasaki,1 Masaro Kumazawa,1 Shuichiro Murakami,1 Shinji Takenaka,1 Kenzo Koike,2 and Kenji Aoki1*

Department of Applied Biological Chemistry, Graduate School of Agricultural Science, Kobe University, Rokko, Kobe 657-8501,1 Beauty Care Research Center, Kao Corporation, Bunka, Sumida-ku, Tokyo 131-8501, Japan2

Received 29 January 2008/ Accepted 18 June 2008

Ceriporiopsis sp. strain MD-1, isolated from forest soil, produced several extracellular enzymes that decolorized human hair melanin. Among them, three enzymes (E1, E2-1, and E2-2) were purified to homogeneity and characterized. The enzymes required hydrogen peroxide in their enzyme reactions and, typical of other fungal peroxidases, oxidized various phenol compounds such as guaiacol, but not 3,4-dimethoxybenzyl alcohol. The spectra of the three enzymes showed an absorption maximum at 406 nm, indicating that they were heme proteins. However, the A406/A280 values of the enzymes were below 0.4, which was lower than those of other peroxidases. E2-1 and E2-2 were similar to each other in their molecular and catalytic properties, and they possibly represent products of posttranslational modifications and/or allelic variants of the same gene, mdcA. The corresponding cDNA was cloned and sequenced; the deduced amino acid sequence showed high identities to the manganese peroxidases from other microorganisms. The specific activities and Km values of E2-1 and E2-2 for synthetic and human hair melanins were much higher than those of Phanerochaete chrysosporium manganese peroxidase and lignin peroxidase.

* Corresponding author. Mailing address: Graduate School of Agricultural Science, Kobe University, Rokko, Kobe 657-8501, Japan. Phone: 81-78-803-5891. Fax: 81-78-882-0481. E-mail: kaoki{at}kobe-u.ac.jp

{triangledown} Published ahead of print on 27 June 2008.

Applied and Environmental Microbiology, August 2008, p. 5106-5112, Vol. 74, No. 16
0099-2240/08/$08.00+0     doi:10.1128/AEM.00253-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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