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Applied and Environmental Microbiology, August 2008, p. 4666-4670, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00092-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Specific Interaction of the Unmodified Bacteriocin Lactococcin 972 with the Cell Wall Precursor Lipid II{triangledown}

Beatriz Martínez,1* Tim Böttiger,2 Tanja Schneider,2 Ana Rodríguez,1 Hans-Georg Sahl,2 and Imke Wiedemann2

Instituto de Productos Lácteos de Asturias, IPLA-CSIC, 33300 Villaviciosa, Asturias, Spain,1 Institut für Medizinische Mikrobiologie und Immunologie-Pharmazeutische Mikrobiologie-Universität Bonn, D-53115 Bonn, Germany2

Received 11 January 2008/ Accepted 21 February 2008

Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipid II antagonized Lcn972 inhibitory activity in vivo. Likewise, Lcn972 only coprecipitated with lipid II micelles. This bacteriocin inhibited the in vitro polymerization of lipid II by the recombinant S. aureus PBP2 and the addition to lipid II of the first glycine catalyzed by FemX. These experiments demonstrate that Lcn972 specifically interacts with lipid II, the substrate of both enzymes. In the presence of Lcn972, nisin pore formation was partially hindered in whole cells. However, binding of Lcn972 to lipid II could not compete with nisin in lipid II-doped 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes, possibly indicating a distinct binding site. The existence of a putative cotarget for Lcn972 activity is discussed in the context of its narrow inhibitory spectrum and the localized action at the division septum. To our knowledge, this is the first unmodified bacteriocin that binds to the cell wall precursor lipid II.

* Corresponding author. Mailing address: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Carretera de Infiesto s/n Apdo. 85, 33300 Villaviciosa, Spain. Phone: 34 985 89 33 59. Fax: 34 985 89 22 33. E-mail: bmf1{at}ipla.csic.es

{triangledown} Published ahead of print on 6 June 2008.

Applied and Environmental Microbiology, August 2008, p. 4666-4670, Vol. 74, No. 15
0099-2240/08/$08.00+0     doi:10.1128/AEM.00092-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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