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Applied and Environmental Microbiology, January 2005, p. 105-113, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.105-113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification and Partial Characterization of the Nonribosomal Peptide Synthetase Gene Responsible for Cereulide Production in Emetic Bacillus cereus

Monika Ehling-Schulz,1 Natasa Vukov,1 Anja Schulz,2 Ranad Shaheen,3 Maria Andersson,3 Erwin Märtlbauer,2 and Siegfried Scherer1*

Microbial Ecology Group, Department of Biosciences, WZW, Technische Universität München, Freising,1 Institute of Hygiene and Technology of Food of Animal Origin, Ludwig-Maximilians-Universität München, Oberschleissheim, Germany,2 Department of Applied Chemistry and Microbiology, College of Agriculture and Forestry at the University of Helsinki, Helsinki, Finland3

Received 10 May 2004/ Accepted 10 August 2004

Cereulide, a depsipeptide structurally related to valinomycin, is responsible for the emetic type of gastrointestinal disease caused by Bacillus cereus. Due to its chemical structure, (D-O-Leu-D-Ala-L-O-Val-L-Val)3, cereulide might be synthesized nonribosomally. Therefore, degenerate PCR primers targeted to conserved sequence motifs of known nonribosomal peptide synthetase (NRPS) genes were used to amplify gene fragments from a cereulide-producing B. cereus strain. Sequence analysis of one of the amplicons revealed a DNA fragment whose putative gene product showed significant homology to valine activation NRPS modules. The sequences of the flanking regions of this DNA fragment revealed a complete module that is predicted to activate valine, as well as a putative carboxyl-terminal thioesterase domain of the NRPS gene. Disruption of the peptide synthetase gene by insertion of a kanamycin cassette through homologous recombination produced cereulide-deficient mutants. The valine-activating module was highly conserved when sequences from nine emetic B. cereus strains isolated from diverse geographical locations were compared. Primers were designed based on the NRPS sequence, and the resulting PCR assay, targeting the ces gene, was tested by using a panel of 143 B. cereus group strains and 40 strains of other bacterial species showing PCR bands specific for only the cereulide-producing B. cereus strains.

* Corresponding author. Mailing address: Microbial Ecology Group, Department of Biosciences, WZW, Technische Universität München, D-85354 Freising, Germany. Phone: 08161-713512. Fax: 08161-714512. E-mail: Siegfried.Scherer{at}wzw.tum.de.

Applied and Environmental Microbiology, January 2005, p. 105-113, Vol. 71, No. 1
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.1.105-113.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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