This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burton, J. P.
Right arrow Articles by Reid, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burton, J. P.
Right arrow Articles by Reid, G.
Agricola
Right arrow Articles by Burton, J. P.
Right arrow Articles by Reid, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2003, p. 97-101, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.97-101.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved Understanding of the Bacterial Vaginal Microbiota of Women before and after Probiotic Instillation

Jeremy P. Burton,1,2* Peter A. Cadieux,2 and Gregor Reid1,2

Canadian Research and Development Centre for Probiotics, The Lawson Health Research Institute,1 Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada2

Received 26 June 2002/ Accepted 24 September 2002

The vaginal bacterial microbiota of 19 premenopausal women was examined by PCR-denaturing gradient gel electrophoresis (DGGE) and sequencing of the V2-V3 region of the 16S rRNA gene. Ten of the women were studied further to investigate the effect and persistence of vaginally inserted capsules containing viable lactobacilli. PCR-DGGE indicated that most subjects had a microbiota represented by one to three dominant DNA fragments. Analysis of these fragments revealed that 79% of the women possessed sequences with high levels of similarity to Lactobacillus species sequences. Sequences homologous to Lactobacillus iners sequences were the most common and were detected in 42% of the women tested. Alteration of the vaginal microbiota could be detected by PCR-DGGE in several women after the instillation of lactobacilli. Additionally, randomly amplified polymorphic DNA analysis of lactobacilli isolated from selective media demonstrated that the exogenous strains could be detected for up to 21 days in some subjects. This study demonstrates that non-culture-based techniques, such as PCR-DGGE, are useful adjuncts for studies of the vaginal microbiota.

* Corresponding author. Mailing address: Lawson Health Research Institute, St. Joseph's Hospital, 268 Grosvenor St., London, Ontario N6A 4V2, Canada. Phone: (519) 646-6100, ext. 65120. Fax: (519) 646-6110. E-mail: jburton{at}lri.sjhc.london.on.ca.

Applied and Environmental Microbiology, January 2003, p. 97-101, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.97-101.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • de Vrese, M. (2009). Health benefits of probiotics and prebiotics in women. Menopause Int 15: 35-40 [Abstract] [Full Text]  
  • Kirjavainen, P. V., Pautler, S., Baroja, M. L., Anukam, K., Crowley, K., Carter, K., Reid, G. (2009). Abnormal Immunological Profile and Vaginal Microbiota in Women Prone to Urinary Tract Infections. CVI 16: 29-36 [Abstract] [Full Text]  
  • Lebeer, S., Vanderleyden, J., De Keersmaecker, S. C. J. (2008). Genes and Molecules of Lactobacilli Supporting Probiotic Action. Microbiol. Mol. Biol. Rev. 72: 728-764 [Abstract] [Full Text]  
  • Spurbeck, R. R., Arvidson, C. G. (2008). Inhibition of Neisseria gonorrhoeae Epithelial Cell Interactions by Vaginal Lactobacillus Species. Infect. Immun. 76: 3124-3130 [Abstract] [Full Text]  
  • Vitali, B., Pugliese, C., Biagi, E., Candela, M., Turroni, S., Bellen, G., Donders, G. G. G., Brigidi, P. (2007). Dynamics of Vaginal Bacterial Communities in Women Developing Bacterial Vaginosis, Candidiasis, or No Infection, Analyzed by PCR-Denaturing Gradient Gel Electrophoresis and Real-Time PCR. Appl. Environ. Microbiol. 73: 5731-5741 [Abstract] [Full Text]  
  • Falagas, M. E., Betsi, G. I., Athanasiou, S. (2006). Probiotics for prevention of recurrent vulvovaginal candidiasis: a review. J Antimicrob Chemother 58: 266-272 [Abstract] [Full Text]  
  • Power, D. A., Burton, J. P., Chilcott, C. N., Tagg, J. R., Dawes, P. J. (2005). Non-Culture-Based Analysis of Bacterial Populations from Patients with Chronic Rhinosinusitis. J. Clin. Microbiol. 43: 5822-5824 [Abstract] [Full Text]  
  • Hyman, R. W., Fukushima, M., Diamond, L., Kumm, J., Giudice, L. C., Davis, R. W. (2005). Microbes on the human vaginal epithelium. Proc. Natl. Acad. Sci. USA 102: 7952-7957 [Abstract] [Full Text]  
  • Zhou, X., Bent, S. J., Schneider, M. G., Davis, C. C., Islam, M. R., Forney, L. J. (2004). Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods. Microbiology 150: 2565-2573 [Abstract] [Full Text]  
  • Burton, J. P., Devillard, E., Cadieux, P. A., Hammond, J.-A., Reid, G. (2004). Detection of Atopobium vaginae in Postmenopausal Women by Cultivation-Independent Methods Warrants Further Investigation. J. Clin. Microbiol. 42: 1829-1831 [Abstract] [Full Text]  
  • Reid, G., Jass, J., Sebulsky, M. T., McCormick, J. K. (2003). Potential Uses of Probiotics in Clinical Practice. Clin. Microbiol. Rev. 16: 658-672 [Abstract] [Full Text]  
  • Reid, G, Bruce, A W (2003). Urogenital infections in women: can probiotics help?. Postgrad. Med. J. 79: 428-432 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»