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Applied and Environmental Microbiology, January 2003, p. 367-372, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.367-372.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Labeling Study To Elucidate the Biosynthesis of 4-(4-Hydroxyphenyl)-Butan-2-one (Raspberry Ketone) by Nidula niveo-tomentosa

H. Zorn, M. Fischer-Zorn, and R. G. Berger*

Zentrum Angewandte Chemie, Universität Hannover, Institut für Lebensmittelchemie, D-30453 Hannover, Germany

Received 27 August 2002/ Accepted 25 October 2002

Submerged cells of the basidiomycete Nidula niveo-tomentosa, a microbial producer of 4-(4-hydroxyphenyl)-butan-2-one, were supplemented with 13C-labeled L-phenylalanines and with [1-13C]glucose. Labeled transformation products were detected by a novel method of analyzing stable isotope-labeled metabolites, gas chromatography (GC) coupled to an atomic emission detector, and by GC-mass spectrometry. A benzoate moiety was side chain elongated according to the poly-ß-keto scheme. The presence of an acetyl coenzyme A-carboxylase inhibitor shifted the spectrum of products to benzyl compounds. Hence, the fungal pathway differs from the one established for plant tissues.

* Corresponding author. Mailing address: Zentrum Angewandte Chemie, Universität Hannover, Institut für Lebensmittelchemie, Wunstorfer Straße 14, D-30453 Hannover, Germany. Phone: 49511 762 4581. Fax: 49511 762 4547. E-mail: rg.berger{at}lci.uni-hannover.de.

Applied and Environmental Microbiology, January 2003, p. 367-372, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.367-372.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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