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Applied and Environmental Microbiology, February 2000, p. 671-677, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cytochrome c3 Mutants of Desulfovibrio desulfuricans

Barbara J. Rapp-Giles,1 Laurence Casalot,1 R. Samuel English,2 Joseph A. Ringbauer Jr.,1 Alain Dolla,3 and Judy D. Wall1,*

Biochemistry Department, University of Missouri---Columbia, Columbia, Missouri 65211,1 College of Health Sciences, Roanoke, Virginia 24016,2 and BIP-IBSM, Centre National de la Recherche Scientifique, 13402 Marseilles Cedex 20, France3

Received 19 August 1999/Accepted 24 November 1999

To explore the physiological role of tetraheme cytochrome c3 in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20, the gene encoding the preapoprotein was cloned, sequenced, and mutated by plasmid insertion. The physical analysis of the DNA from the strain carrying the integrated plasmid showed that the insertion was successful. The growth rate of the mutant on lactate with sulfate was comparable to that of the wild type; however, mutant cultures did not achieve the same cell densities. Pyruvate, the oxidation product of lactate, served as a poor electron source for the mutant. Unexpectedly, the mutant was able to grow on hydrogen-sulfate medium. These data support a role for tetraheme cytochrome c3 in the electron transport pathway from pyruvate to sulfate or sulfite in D. desulfuricans G20.

* Corresponding author. Mailing address: Biochemistry Department, 117 Schweitzer Hall, University of Missouri---Columbia, Columbia, MO 65211. Phone: (573) 882-8726. Fax: (573) 882-5635. E-mail: wallj{at}missouri.edu.

Applied and Environmental Microbiology, February 2000, p. 671-677, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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