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Applied and Environmental Microbiology, February 2000, p. 524-528, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Natural Mediators in the Oxidation of Polycyclic Aromatic Hydrocarbons by Laccase Mediator Systems

Christian Johannes and Andrzej Majcherczyk*

Institute of Forest Botany, University of Göttingen, 37077 Göttingen, Germany

Received 15 September 1999/Accepted 3 November 1999

The oxidation of polycyclic aromatic compounds was studied in systems consisting of laccase from Trametes versicolor and so-called mediator compounds. The enzymatic oxidation of acenaphthene, acenaphthylene, anthracene, and fluorene was mediated by various laccase substrates (phenols and aromatic amines) or compounds produced and secreted by white rot fungi. The best natural mediators, such as phenol, aniline, 4-hydroxybenzoic acid, and 4-hydroxybenzyl alcohol were as efficient as the previously described synthetic compounds ABTS [2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] and 1-hydroxybenzotriazole. The oxidation efficiency increased proportionally with the redox potentials of the phenolic mediators up to a maximum value of 0.9 V and decreased thereafter with redox potentials exceeding this value. Natural compounds such as methionine, cysteine, and reduced glutathione, containing sulfhydryl groups, were also active as mediator compounds.

* Corresponding author. Mailing address: Institut für Forstbotanik, Georg-August-Universität Göttingen, Büsgenweg 2, 37077 Göttingen, Germany. Phone: 551 39 94 82. Fax: 551 39 27 05. E-mail: amajche{at}gwdg.de.

Applied and Environmental Microbiology, February 2000, p. 524-528, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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