AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Annweiler, E.
Right arrow Articles by Meckenstock, R. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Annweiler, E.
Right arrow Articles by Meckenstock, R. U.
Agricola
Right arrow Articles by Annweiler, E.
Right arrow Articles by Meckenstock, R. U.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2000, p. 5329-5333, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Anaerobic Degradation of 2-Methylnaphthalene by a Sulfate-Reducing Enrichment Culture†

Eva Annweiler,1 Arne Materna,2 Michael Safinowski,2 Andreas Kappler,2 Hans H. Richnow,3 Walter Michaelis,1 and Rainer U. Meckenstock2,*

Institut für Biogeochemie und Meereschemie, Universität Hamburg, D-20146 Hamburg,1 UFZ, Umweltforschungszentrum Leipzig-Halle GmbH, D-04318 Leipzig,3 and Fakultät für Biologie, Universität Konstanz, D-78457 Konstanz,2 Germany

Received 26 June 2000/Accepted 29 September 2000

Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid by comparison with chemically synthesized reference compounds. Naphthyl-2-methyl-succinic acid accumulated to 0.5 µM in culture supernatants. Production of naphthyl-2-methyl-succinic acid was analyzed in enzyme assays with dense cell suspensions. The conversion of 2-methylnaphthalene to naphthyl-2-methyl-succinic acid was detected at a specific activity of 0.020 ± 0.003 nmol min-1 mg of protein-1 only in the presence of cells and fumarate. We conclude that under anaerobic conditions 2-methylnaphthalene is activated by fumarate addition to the methyl group, as is the case in anaerobic toluene degradation. The second group of metabolites comprised 2-naphthoic acid and reduced 2-naphthoic acid derivatives, including 5,6,7,8-tetrahydro-2-naphthoic acid, octahydro-2-naphthoic acid, and decahydro-2-naphthoic acid. These compounds were also identified in an earlier study as products of anaerobic naphthalene degradation with the same enrichment culture. A pathway for anaerobic degradation of 2-methylnaphthalene analogous to that for anaerobic toluene degradation is proposed.

* Corresponding author. Mailing address: Fakultät für Biologie, Universität Konstanz, Universitätsstr. 10, D-78457 Konstanz, Germany. Phone: 49-7531-884541. Fax: 49-7531-882966. E-mail: rainer.meckenstock{at}uni-konstanz.de.

dagger Publication 101 of the Deutsche Forschungsgemeinschaft priority program 546, Geochemical Processes with Long-Term Effects in Anthropogenically Affected Seepage and Groundwater.

Applied and Environmental Microbiology, December 2000, p. 5329-5333, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.