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Applied and Environmental Microbiology, November 2000, p. 4720-4724, Vol. 66, No. 11
0099-2240/00/$04.00+0

Characterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Matthew L. Magnuson,1,* James H. Owens,2 and Catherine A. Kelty1

Treatment Technology Evaluation Branch1 and Microbial Contaminants Control Branch,2 National Risk Management Research Laboratory, Water Supply and Water Resources Division, Office of Research and Development, United States Environmental Protection Agency, Cincinnati, Ohio 45268

Received 26 June 2000/Accepted 15 August 2000

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns for C. parvum were distinguishable from those obtained for Cryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.

* Corresponding author. Mailing address: U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268. Phone: (513) 569-7321. Fax: (513) 569-7658. E-mail: Magnuson.Matthew{at}epa.gov.

Applied and Environmental Microbiology, November 2000, p. 4720-4724, Vol. 66, No. 11
0099-2240/00/$04.00+0


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