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Applied and Environmental Microbiology, October 2000, p. 4595-4597, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibacterial Activity Evaluation of Microcin J25 against Diarrheagenic Escherichia coli

Sophie Sable, Anne-Marie Pons, Sandrine Gendron-Gaillard, and Gilles Cottenceau*

Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences, Université de La Rochelle, La Rochelle, France

Received 23 February 2000/Accepted 7 July 2000

The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 µg ml-1, respectively. A 104-CFU ml-1 contamination by this strain was destroyed in milk and meat extract by 6.25 µg of J25 ml-1 and in half-diluted egg yolk by 50 µg of J25 ml-1.

* Corresponding author. Mailing address: LGPC, Pôle Sciences, Université de La Rochelle, Avenue Marillac, 17042 La Rochelle, France. Phone: 33-546-458-246. Fax: 33-546-458-247. E-mail: gilles.cottenceau{at}univ-lr.fr.

Applied and Environmental Microbiology, October 2000, p. 4595-4597, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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