AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lampel, K. A.
Right arrow Articles by Kornegay, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lampel, K. A.
Right arrow Articles by Kornegay, L.
Agricola
Right arrow Articles by Lampel, K. A.
Right arrow Articles by Kornegay, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2000, p. 4539-4542, Vol. 66, No. 10
0099-2240/00/$04.00+0

Improved Template Preparation for PCR-Based Assays for Detection of Food-Borne Bacterial Pathogens

Keith A. Lampel,* Palmer A. Orlandi, and Leroy Kornegay

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204

Received 22 March 2000/Accepted 30 June 2000

Shigella flexneri, Salmonella enterica serotype Typhimurium, and Listeria monocytogenes were applied to FTA filters, and the filters were used directly as templates to demonstrate their sensitivity and applicability in PCR-based detection assays. With pure cultures, the sensitivities of detection by FTA filter-based PCR were 30 to 50 and 200 CFU for the gram-negative enterics and Listeria, respectively. Different numbers of S. flexneri cells were used in controlled contamination experiments with several different foods (produce, beef, and apple cider). Aliquots from concentrated food washes subsequently spotted onto FTA filters and assayed by PCR gave consistently positive results and detection limits similar to those observed with pure-culture dilutions. This universal method for PCR template preparation from bacterial cells is rapid and highly sensitive and reduces interference from food-associated inhibitors of PCR. In addition, its broad applicability eliminates the need for multiple methods for analysis of food matrices.

* Corresponding author. Mailing address: Food and Drug Administration, HFS-237, 200 C St., SW, Washington, DC 20204. Phone: (202) 205-4515. Fax: (202) 205-4939. E-mail: kal{at}codon.nih.gov.

Applied and Environmental Microbiology, October 2000, p. 4539-4542, Vol. 66, No. 10
0099-2240/00/$04.00+0


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.