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Applied and Environmental Microbiology, September 1999, p. 4276-4279, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Efficacy of Electrolyzed Oxidizing Water for Inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes

Kumar S. Venkitanarayanan,1 Gabriel O. Ezeike,2 Yen-Con Hung,2 and Michael P. Doyle2,*

Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269,1 and Center for Food Safety and Quality Enhancement, College of Agricultural and Environmental Sciences, University of Georgia, Griffin, Georgia 30223-17972

Received 14 December 1998/Accepted 18 June 1999

The efficacy of electrolyzed oxidizing water for inactivating Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes was evaluated. A five-strain mixture of E. coli O157:H7, S. enteritidis, or L. monocytogenes of approximately 108 CFU/ml was inoculated in 9 ml of electrolyzed oxidizing water (treatment) or 9 ml of sterile, deionized water (control) and incubated at 4 or 23°C for 0, 5, 10, and 15 min; at 35°C for 0, 2, 4, and 6 min; or at 45°C for 0, 1, 3, and 5 min. The surviving population of each pathogen at each sampling time was determined on tryptic soy agar. At 4 or 23°C, an exposure time of 5 min reduced the populations of all three pathogens in the treatment samples by approximately 7 log CFU/ml, with complete inactivation by 10 min of exposure. A reduction of >= 7 log CFU/ml in the levels of the three pathogens occurred in the treatment samples incubated for 1 min at 45°C or for 2 min at 35°C. The bacterial counts of all three pathogens in control samples remained the same throughout the incubation at all four temperatures. Results indicate that electrolyzed oxidizing water may be a useful disinfectant, but appropriate applications need to be validated.

* Corresponding author. Mailing address: Center for Food Safety and Quality Enhancement, College of Agricultural and Environmental Sciences, University of Georgia, 1109 Experiment St., Griffin, GA 30223-1797. Phone: (770) 228-7284. Fax: (770) 229-3216. E-mail: mdoyle{at}cfsqe.griffin.peachnet.edu.

Applied and Environmental Microbiology, September 1999, p. 4276-4279, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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