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Applied and Environmental Microbiology, September 1999, p. 3908-3914, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Ribotyping, Randomly Amplified Polymorphic DNA Analysis, and Pulsed-Field Gel Electrophoresis in Typing of Lactobacillus rhamnosus and L. casei Strains

Soile Tynkkynen,1,* Reetta Satokari,2 Maria Saarela,2 Tiina Mattila-Sandholm,2 and Maija Saxelin1

Valio Ltd. Research and Development Centre,1 FIN-00039 Valio, and VTT Biotechnology and Food Research, FIN-02044 VTT,2 Finland

Received 10 November 1998/Accepted 27 May 1999

A total of 24 strains, biochemically identified as members of the Lactobacillus casei group, were identified by PCR with species-specific primers. The same set of strains was typed by randomly amplified polymorphic DNA (RAPD) analysis, ribotyping, and pulsed-field gel electrophoresis (PFGE) in order to compare the discriminatory power of the methods. Species-specific primers for L. rhamnosus and L. casei identified the type strain L. rhamnosus ATCC 7469 and the neotype strain L. casei ATCC 334, respectively, but did not give any signal with the recently revived species L. zeae, which contains the type strain ATCC 15820 and the strain ATCC 393, which was previously classified as L. casei. Our results are in accordance with the suggested new classification of the L. casei group. Altogether, 21 of the 24 strains studied were identified with the species-specific primers. In strain typing, PFGE was the most discriminatory method, revealing 17 genotypes for the 24 strains studied. Ribotyping and RAPD analysis yielded 15 and 12 genotypes, respectively.

* Corresponding author. Mailing address: Valio Ltd. Research and Development Centre, P.O. Box 30, FIN-00039 Valio, Finland. Phone: 358 10381 3125. Fax: 358 10381 3129. E-mail: soile.tynkkynen{at}valio.fi.

Applied and Environmental Microbiology, September 1999, p. 3908-3914, Vol. 65, No. 9
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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