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Applied and Environmental Microbiology, August 1999, p. 3319-3324, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Anaerobic Mineralization of Quaternary Carbon Atoms: Isolation of Denitrifying Bacteria on Dimethylmalonate

Olaf Kniemeyer,1 Christina Probian,1 Ramon Rosselló-Mora,2 and Jens Harder1,*

Department of Microbiology1 and Molecular Ecology Group,2 Max-Planck-Institute for Marine Microbiology, D-28359 Bremen, Germany

Received 19 January 1999/Accepted 18 May 1999

The microbial capacity to degrade simple organic compounds with quaternary carbon atoms was demonstrated by enrichment and isolation of five denitrifying strains on dimethylmalonate as the sole electron donor and carbon source. Quantitative growth experiments showed a complete mineralization of dimethylmalonate. According to phylogenetic analysis of the complete 16S rRNA genes, two strains isolated from activated sewage sludge were related to the genus Paracoccus within the alpha -Proteobacteria (98.0 and 98.2% 16S rRNA gene similarity to Paracoccus denitrificansT), and three strains isolated from freshwater ditches were affiliated with the beta -Proteobacteria (97.4 and 98.3% 16S rRNA gene similarity to Herbaspirillum seropedicaeT and Acidovorax facilisT, respectively). Most-probable-number determinations for denitrifying populations in sewage sludge yielded 4.6 × 104 dimethylmalonate-utilizing cells ml-1, representing up to 0.4% of the total culturable nitrate-reducing population.

* Corresponding author. Mailing address: Abteilung Mikrobiologie, Max-Planck-Institut für marine Mikrobiologie, Celsiusstr. 1, D-28359 Bremen, Germany. Phone: 49-421-2028-750. Fax: 49-421-2028-580. E-mail: jharder{at}mpi-bremen.de.

Applied and Environmental Microbiology, August 1999, p. 3319-3324, Vol. 65, No. 8
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.