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Applied and Environmental Microbiology, July 1999, p. 3226-3228, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Specific Detection of the Gene for the Extracellular Neutral Protease of Bacillus cereus by PCR and Blot Hybridization

H.-J. Bach,1,* D. Errampalli,2 K. T. Leung,2 H. Lee,2 A. Hartmann,1 J. T. Trevors,2 and J. C. Munch1

Institute for Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany,1 and Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W12

Received 14 December 1998/Accepted 6 April 1999

A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus, B. mycoides, and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

* Corresponding author. Mailing address: GSF-National Research Center for Environment and Health, Institute for Soil Ecology, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany. Phone: 49 (89) 3187-2478. Fax: 49 (89) 3187-3376. E-mail: bach{at}gsf.de.

Applied and Environmental Microbiology, July 1999, p. 3226-3228, Vol. 65, No. 7
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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