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Applied and Environmental Microbiology, April 1999, p. 1794-1797, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance

Y. Manor,1 R. Handsher,1 T. Halmut,1 M. Neuman,1 B. Abramovitz,1 A. Mates,2 and E. Mendelson1,*

Central Virology Laboratory, Public Health Laboratories, Chaim Sheba Medical Center, Tel-Hashomer,1 and Department of Laboratories, Ministry of Health, Jerusalem,2 Israel

Received 20 May 1998/Accepted 28 January 1999

We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.

* Corresponding author. Mailing address: Central Virology Laboratory, Chaim Sheba Medical Center, Tel-Hashomer 52621, Israel. Phone: 972-3-530-2421. Fax: 972-3-530-2457. E-mail: ellamen{at}ibm.net.

Applied and Environmental Microbiology, April 1999, p. 1794-1797, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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