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Applied and Environmental Microbiology, April 1999, p. 1781-1785, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Different Strategies for Molecular Differentiation of Mycobacterium bovis Strains Isolated in Sardinia, Italy

Leonardo A. Sechi,1,* Guido Leori,2 Stefano A. Lollai,2 Ilaria Duprè,1 Paola Molicotti,1 Giovanni Fadda,3 and Stefania Zanetti1

Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari,1 and Istituto Zooprofilattico Sperimantale della Sardegna,2 07100 Sassari, and Istituto di Microbiologia, Facoltà di Medicina e Chirurgia "Agostino Gemelli," Università Cattolica del Sacro Cuore, 00168 Rome,3 Italy

Received 4 December 1998/Accepted 28 January 1999

Different genetic markers were used to analyze 22 Mycobacterium bovis strains isolated from cattle in Sardinia and one human isolate. IS6110 DNA fingerprinting differentiated the strains into six patterns, whereas with enterobacterial repetitive consensus sequence primers produced seven clusters. PCR ribotyping followed by digestion with HaeIII and PvuII produced five and seven patterns, respectively. PCR with the (GTG)5 oligonucleotide primer showed the best discriminatory power, generating eight clusters among the strains analyzed.

* Corresponding author. Mailing address: Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy. Phone: 079 228303. Fax: 079 212345. E-mail: sechila{at}ssmain.uniss.it.

Applied and Environmental Microbiology, April 1999, p. 1781-1785, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.