AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tholosan, O.
Right arrow Articles by Bianchi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tholosan, O.
Right arrow Articles by Bianchi, A.
GeoRef
Right arrow GeoRef Citation
Agricola
Right arrow Articles by Tholosan, O.
Right arrow Articles by Bianchi, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 1999, p. 1619-1626, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Biphasic Extracellular Proteolytic Enzyme Activity in Benthic Water and Sediment in the Northwestern Mediterranean Sea

Olivier Tholosan,1 François Lamy,1 Jean Garcin,1 Thalia Polychronaki,2 and Armand Bianchi1,*

Microbiologie Marine, Centre National de la Recherche Scientifique-Institut National des Sciences de l'Univers, Université de la Méditerranée, F-13288 Marseille Cedex 9, France,1 and Institute of Marine Biology of Crete, GR71003, Iraklion, Crete, Greece2

Received 4 November 1998/Accepted 26 January 1999

In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (Vm) and affinities (Km) of natural benthic microbial communities by the multiconcentration kinetic method. This investigation was performed during the winter and summer of 1997 with a set of 36 samples of near-bottom water and sediment collected from a coastal area and an offshore area in the western part of the Gulf of Lions. The existence of biphasic microbial ectoproteolysis was statistically confirmed for both the near-bottom water and the sediment, regardless of the spatial and seasonal conditions. Globally, 72.2% of the entire set of bacterial consortia collected at the water-sediment boundary layer showed biphasic microbial kinetics. A specific estimator of the biphasicity indicated that deep benthic bacterial consortia responded better with episodic nutrient supplies than shallower benthic bacterial consortia responded.

* Corresponding author. Mailing address: Microbiologie Marine, Centre National de la Recherche Scientifique-Institut National des Sciences de l'Univers-EP 2032, Université de la Méditerranée, F-13288 Marseille Cedex 9, France. Phone: 33 4 91 82 90 49. Fax: 33 4 91 82 90 51. E-mail: a-bianchi{at}luminy.univ-mrs.fr.

Applied and Environmental Microbiology, April 1999, p. 1619-1626, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.