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Applied and Environmental Microbiology, April 1999, p. 1596-1602, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cloning and Partial Characterization of Endopolygalacturonase Genes from Botrytis cinerea

J. P. Wubben,1 W. Mulder,1 A. ten Have,2 J. A. L. van Kan,2 and J. Visser1,*

Section of Molecular Genetics of Industrial Micro-organisms, Wageningen Agricultural University, 6703 HA Wageningen,1 and Laboratory of Phytopathology, Wageningen Agricultural University, 6709 PD Wageningen,2 The Netherlands

Received 3 August 1998/Accepted 12 January 1999

Botrytis cinerea is a plant-pathogenic fungus infecting over 200 different plant species. We use a molecular genetic approach to study the process of pectin degradation by the fungus. Recently, we described the cloning and characterization of an endopolygalacturonase (endoPG) gene from B. cinerea (Bcpg1) which is required for full virulence. Here we describe the cloning and characterization of five additional endoPG-encoding genes from B. cinerea SAS56. The identity at the amino acid level between the six endoPGs of B. cinerea varied from 34 to 73%. Phylogenetic analysis, by using a group of 35 related fungal endoPGs and as an outgroup one plant PG, resulted in the identification of five monophyletic groups of closely related proteins. The endoPG proteins from B. cinerea SAS56 could be assigned to three different monophyletic groups. DNA blot analysis revealed the presence of the complete endoPG gene family in other strains of B. cinerea, as well as in other Botrytis species. Differential gene expression of the gene family members was found in mycelium grown in liquid culture with either glucose or polygalacturonic acid as the carbon source.

* Corresponding author. Mailing address: Section of Molecular Genetics of Industrial Micro-organisms, Wageningen Agricultural University, Dreijenlaan 2, 6703 HA Wageningen, The Netherlands. Phone: 31-317-482865 or 31-317-484439. Fax: 31-317-484011. E-mail: office{at}algemeen.mgim.wau.nl.

Applied and Environmental Microbiology, April 1999, p. 1596-1602, Vol. 65, No. 4
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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