AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jordan, S. L.
Right arrow Articles by Park, S. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jordan, S. L.
Right arrow Articles by Park, S. F.
Agricola
Right arrow Articles by Jordan, S. L.
Right arrow Articles by Park, S. F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 1999, p. 1308-1311, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Augmentation of Killing of Escherichia coli O157 by Combinations of Lactate, Ethanol, and Low-pH Conditions

Sarah L. Jordan,1,* Jayne Glover,2 Laura Malcolm,2 Fiona M. Thomson-Carter,3 Ian R. Booth,2 and Simon F. Park1,dagger

Institute of Food Research, Reading Laboratory, Reading, RG6 6BZ,1 and Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences,2 and Scottish Reference Laboratory, Aberdeen Royal Hospitals Trust,3 Foresterhill, Aberdeen, AB25 2ZD, United Kingdom

Received 30 July 1998/Accepted 8 December 1998

The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.

* Corresponding author. Mailing address: Institute of Food Research, Reading Laboratory, Earley Gate, Reading RG6 6BZ, United Kingdom. Phone: 44-118-935-7228. Fax: 44-118-935-7222. E-mail: sarah.jordan{at}bbsrc.ac.uk.

dagger Present address: School of Biological Sciences, University of Surrey, Guildford, GU2 5XH, United Kingdom.

Applied and Environmental Microbiology, March 1999, p. 1308-1311, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.