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Applied and Environmental Microbiology, March 1999, p. 1175-1179, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Flagellin Genes from Clinical and Environmental Pseudomonas aeruginosa Isolates

J. Alun W. Morgan,1,* Nessa F. Bellingham,2 Craig Winstanley,3 Margaret A. Ousley,1 C. Anthony Hart,4 and Jon R. Saunders5

Department of Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF,1 School of Natural and Environmental Sciences, Coventry University, Coventry, CV1 5FB,2 Department of Biomedical Sciences, University of Bradford, Bradford, BD7 1DP,3 Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool, L7 8XP,4 and School of Biological Sciences, University of Liverpool, Liverpool, L69 7ZB,5 United Kingdom

Received 8 September 1998/Accepted 8 December 1998

Pseudomonas aeruginosa, an important opportunistic pathogen, was isolated from environmental samples and compared to clinically derived strains. While P. aeruginosa was isolated readily from an experimental mushroom-growing unit, it was found only rarely in other environmental samples. A flagellin gene PCR-restriction fragment length polymorphism analysis of the isolates revealed that environmental and clinical P. aeruginosa strains are not readily distinguishable. The variation in the central regions of the flagellin genes of seven of the isolates was investigated further. The strains used included two strains with type a genes (998 bp), four strains with type b genes (1,258 bp), and one strain, K979, with a novel flagellin gene (2,199 bp). The route by which flagellin gene variation has occurred in P. aeruginosa is discussed.

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, United Kingdom. Phone: 01789 470382. Fax: 01789 470552. E-mail: alun.morgan{at}hri.ac.uk.

Applied and Environmental Microbiology, March 1999, p. 1175-1179, Vol. 65, No. 3
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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