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Applied and Environmental Microbiology, December 1999, p. 5639-5641, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Enzymatic Function of the Nor-1 Protein in Aflatoxin Biosynthesis in Aspergillus parasiticus

Renqing Zhou and John E. Linz*

Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824-1224

Received 16 April 1999/Accepted 19 September 1999

The nor-1 gene is involved in aflatoxin biosynthesis in Aspergillus parasiticus and was predicted to encode a norsolorinic acid ketoreductase. Recombinant Nor-1 expressed in Escherichia coli converted the 1' keto group of norsolorinic acid to the 1' hydroxyl group of averantin in crude E. coli cell extracts in the presence of NADPH. The results confirm that Nor-1 functions as a ketoreductase in vitro.

* Corresponding author. Mailing address: Department of Food Science and Human Nutrition, 234B GM Trout Food Science and Human Nutrition Building, Michigan State University, E. Lansing, MI 48824-1224. Phone: (517) 353-9624. Fax: (517) 353-8963. E-mail: jlinz{at}pilot.msu.edu.

Applied and Environmental Microbiology, December 1999, p. 5639-5641, Vol. 65, No. 12
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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