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Applied and Environmental Microbiology, November 1999, p. 5173-5176, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A New Selective Medium for Bifidobacterium spp.

Y. Nebra* and A. R. Blanch

Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, E-08028 Barcelona, Spain

Received 10 February 1999/Accepted 7 August 1999

A new selective antibiotic-free medium for Bifidobacterium spp. is defined. This medium has lactulose as the main carbon source and includes methylene blue, propionic acid, and lithium chloride as inhibitors of some related bacterial species. The low pH of the medium contributes to the inhibition of the growth of Enterobacteriaceae. This new selective medium has a simple composition, and the level of recovery it yields is similar to those yielded by nonselective media for Bifidobacterium strains. It could thus be used for routine analysis in environmental or food microbiology.

* Corresponding author. Mailing address: Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal, 645, E-08028 Barcelona, Spain. Phone: 34 93 402 14 39. Fax: 34 93 411 05 92. E-mail: ynebra{at}porthos.bio.ub.es.

Applied and Environmental Microbiology, November 1999, p. 5173-5176, Vol. 65, No. 11
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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