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Appl Environ Microbiol, July 1998, p. 2705-2709, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Lyme Disease Borrelia Species in Northeastern China Resemble Those Isolated from Far Eastern Russia and Japan

Muqing Li,1,2,* Toshiyuki Masuzawa,2 Nobuhiro Takada,3 Fubito Ishiguro,4 Hiromi Fujita,5 Atsue Iwaki,2 Haipeng Wang,6 Jichun Wang,6 Masato Kawabata,1 and Yasutake Yanagihara2

International Center for Medical Research, Kobe University School of Medicine, Kobe, Hyogo 650,1 Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Shizuoka 422,2 Department of Immunology and Medical Zoology, Fukui Medical School, Matsuoka, Fukui 910-11,3 Fukui Prefectural Institute of Public Health, Fukui, Fukui 910,4 and Ohara Research Laboratory, Ohara General Hospital, Fukushima, Fukushima 960,5 Japan, and Department of Parasitology, Chinese Medical University, Shengyang 110001, China6

Received 15 December 1997/Accepted 21 April 1998

Fifty-nine Borrelia burgdorferi sensu lato culture isolates collected from northeastern China were characterized by 5S-23S rRNA intergenic spacer restriction fragment length polymorphism (RFLP) analysis and reactivity with monoclonal antibodies (MAbs). Among 59 culture isolates, 30 (50.8%) were Borrelia garinii and 17 (28.8%) were Borrelia afzelii, 2 were mixtures composed of B. garinii with RFLP pattern B and B. garinii with pattern C, and 9 were mixtures composed of B. garinii and B. afzelii. One isolate, ChY13p, produced a unique pattern and was identified as B. garinii based on analyses of 16S rRNA gene sequence, flagellin PCR-RFLP typing, and MAb reactivities. No Borrelia burgdorferi sensu stricto or Borrelia japonica isolates were detected. The results indicate that Lyme disease Borrelia species in northeastern China resemble those of Borrelia isolates from far eastern Russia and Japan.

* Corresponding author. Mailing address: International Center for Medical Research, Kobe University School of Medicine, Kusunoki-cho 7-5-1, Chuo-ku, Kobe 650, Japan. Phone: 81-78-341-7451, ext. 3560. Fax: 81-78-371-5171. E-mail: muqingl{at}kobe-u.ac.jp.

Appl Environ Microbiol, July 1998, p. 2705-2709, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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