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Appl Environ Microbiol, May 1998, p. 1947-1949, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Cloning of the Ribosomal Protein L41 Gene of Phaffia rhodozyma and Its Use as a Drug Resistance Marker for Transformation

I.-G. Kim,1 S.-K. Nam,1 J.-H. Sohn,1 S.-K. Rhee,1 G.-H. An,2 S.-H. Lee,2 and E.-S. Choi1,*

Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-600,1 and Research and Development Center, Haitai Confectionery Co., Ltd., 255-9, Huam-dong, Yongsan-ku, Seoul 140-190,2 Korea

Received 30 December 1997/Accepted 5 March 1998

The ribosomal protein L41 gene of Phaffia rhodozyma was cloned and used as a dominant selectable marker for cycloheximide resistance in the transformation of P. rhodozyma. Electrotransformation with a plasmid containing a ribosomal DNA fragment as a targeting signal typically yielded 800 to 1,200 transformants/µg of DNA with an integrated copy number of about seven per haploid genome.

* Corresponding author. Mailing address: Applied Microbiology Research Division, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon 305-600, Korea. Phone: 82-42-860-4453. Fax: 82-42-860-4594. E-mail: choi4162{at}kribb4680.kribb.re.kr.

Appl Environ Microbiol, May 1998, p. 1947-1949, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.