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Appl Environ Microbiol, April 1998, p. 1541-1544, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

An Origin of Transfer (oriT) on the Conjugative Element pRS01 from Lactococcus lactis subsp. lactis ML3dagger

David A. Mills,1,Dagger Trevor G. Phister,1,§ Gary M. Dunny,1 and Larry L. McKay2,*

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455-0312,1 and Department of Food Science and Nutrition, University of Minnesota, St. Paul, Minnesota 551082

Received 27 August 1997/Accepted 9 January 1998

Previous analysis of the Tra1 region of the conjugative element pRS01 from Lactococcus lactis subsp. lactis ML3 suggested that an origin of transfer (oriT) was present. Deletion derivatives of this cloned Tra1 region were assayed for mobilization in the presence of the wild-type pRS01 element in trans. The pRS01 oriT was localized to a 446-nucleotide segment in the intergenic region between open reading frames ltrD and ltrE. Sequence analysis of this region revealed a cluster of direct and inverted repeat structures characteristic of oriT regions associated with other conjugative systems.

* Corresponding author. Mailing address: Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St. Paul, MN 55108. Phone: (612) 624-3090. Fax: (612) 625-5272. E-mail: lmckay{at}che2.che.umn.edu.

dagger Paper no. 97-1-18-0024 of the contribution series of the Minnesota Agricultural Experiment Station based on research conducted under Project 18-62.

Dagger Present address: Department of Food Science, North Carolina State University, Raleigh, NC 27695-7624.

§ Present address: Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.