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Appl Environ Microbiol, March 1998, p. 1099-1105, Vol. 64, No. 3
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Root-Associated Methanotrophs from Three Freshwater Macrophytes: Pontederia cordata, Sparganium eurycarpum, and Sagittaria latifoliadagger

A. Calhoun and G. M. King*

Darling Marine Center, University of Maine, Walpole, Maine 04573

Received 15 September 1997/Accepted 8 December 1997

Root-associated methanotrophic bacteria were enriched from three common aquatic macrophytes: Pontederia cordata, Sparganium eurycarpum, and Sagittaria latifolia. At least seven distinct taxa belonging to groups I and II were identified and presumptively assigned to the genera Methylosinus, Methylocystis, Methylomonas, and Methylococcus. Four of these strains appeared to be novel on the basis of partial 16S ribosomal DNA sequence analysis. The root-methanotroph association did not appear to be highly specific, since multiple methanotrophs were isolated from each of the three plant species. Group II methanotrophs were isolated most frequently; though less common, group I isolates accounted for three of the seven distinct methanotrophs. Apparent Km values for methane uptake by representative cultures ranged from 3 to >17 µM; for five of the eight cultures examined, apparent Km values agreed well with apparent Km estimates for plant roots, suggesting that these strains may be representative of those active in situ.

* Corresponding author. Phone: (207) 563-3146 ext. 207. Fax: (207) 563-3119. E-mail: GKing{at}Maine.Maine.Edu.

dagger Contribution 311 from the Darling Marine Center.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.