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Applied and Environmental Microbiology, November 1998, p. 4384-4389, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Characterization of Epiphytic Bacterial Communities on Charophycean Green Algae

Madeline M. Fisher,1,* Lee W. Wilcox,2 and Linda E. Graham2

Department of Agronomy1 and Department of Botany,2 University of Wisconsin, Madison, Wisconsin 53706

Received 11 May 1998/Accepted 31 August 1998

Epiphytic bacterial communities within the sheath material of three filamentous green algae, Desmidium grevillii, Hyalotheca dissiliens, and Spondylosium pulchrum (class Charophyceae, order Zygnematales), collected from a Sphagnum bog were characterized by PCR amplification, cloning, and sequencing of 16S ribosomal DNA. A total of 20 partial sequences and nine different sequence types were obtained, and one sequence type was recovered from the bacterial communities on all three algae. By phylogenetic analysis, the cloned sequences were placed into several major lineages of the Bacteria domain: the Flexibacter/Cytophaga/Bacteroides phylum and the alpha , beta , and gamma  subdivisions of the phylum Proteobacteria. Analysis at the subphylum level revealed that the majority of our sequences were not closely affiliated with those of known, cultured taxa, although the estimated evolutionary distances between our sequences and their nearest neighbors were always less than 0.1 (i.e., greater than 90% similar). This result suggests that the majority of sequences obtained in this study represent as yet phenotypically undescribed bacterial species and that the range of bacterial-algal interactions that occur in nature has not yet been fully described.

* Corresponding author. Mailing address: Department of Agronomy, University of Wisconsin, 1575 Linden Dr., Madison, WI 53706. Phone: (608) 262-6457. Fax: (608) 262-7509. E-mail: mmfisher{at}students.wisc.edu.

Applied and Environmental Microbiology, November 1998, p. 4384-4389, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.