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Applied and Environmental Microbiology, October 1998, p. 3813-3817, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Quantitative and Physiological Analyses of Chloride Dependence of Growth of Halobacillus halophilus

Markus Roeßler and Volker Müller*

Lehrstuhl für Mikrobiologie, Ludwig-Maximilians-Universität München, 80638 Munich, Germany

Received 16 March 1998/Accepted 27 July 1998

A quantitative analysis of the Cl- dependence of growth of Halobacillus halophilus was performed. Optimal growth rates were obtained at Cl- concentrations of between 0.5 and 2.0 M, and the final yield was also strictly dependent on the Cl- concentration. Br- but not I-, SO42-, NO2-, SO2-, OCN-, SCN-, BO2-, or BrO3- could substitute for Cl-. To analyze the function of chloride, chloride concentration was determined. At low external Cl- (Cle-) concentrations, the growth rate was low and Cl- was excluded from the cytoplasm; increasing the Cle- concentration led to an increase in the growth rate and an energy-dependent uptake of Cl-, thus decreasing the Cle-/internal Cli- gradient from >= 10 at 0.1 M Cle- to a nearly constant value of 2 at Cle- concentrations which allowed optimal growth. Two membrane proteins with apparent molecular masses of 31 and 16 kDa which were identified to be specific for Cl--grown cultures are possible candidates for a chloride uptake system.

* Corresponding author. Mailing address: Lehrstuhl für Mikrobiologie, Ludwig-Maximilians-Universität München, Maria-Ward-Str. 1a, 80638 Munich, Germany. Phone: 49-8917919836. Fax: 49-8917919855. E-mail: v.mueller{at}lrz.uni-muenchen.de.

Applied and Environmental Microbiology, October 1998, p. 3813-3817, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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