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Appl Environ Microbiol, January 1998, p. 366-369, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Measurement of Antimicrobial-Resistant Escherichia coli in Pig Feces with a Hydrophobic Grid Membrane Filter Interpreter System

R. Hugo Dunlop,1,dagger Scott A. McEwen,1,* Alan H. Meek,1 Robert C. Clarke,2 Robert M. Friendship,1 William D. Black,3 and Anthony N. Sharpe4

Departments of Population Medicine1 and Biomedical Sciences,3 University of Guelph, and Health of Animals Laboratory, Health Canada,2 Guelph, and Bureau of Microbial Hazards, Health Canada, Tunney's Pasture, Ottawa,4 Ontario, Canada

Received 30 April 1997/Accepted 26 September 1997

Hydrophobic grid membrane filter technology was used to measure resistance among Escherichia coli in pig fecal samples to ampicillin, sulfisoxazole, and tetracycline. The method accurately measured resistance, with sensitivities ranging from 96.5 to 99.5% and specificities ranging from 87.0 to 98.3%, and it identified E. coli with 96% confidence.

* Corresponding author. Mailing address: Department of Population Medicine, University of Guelph, Guelph, Ontario, Canada N1G 2W1. Phone: 519-824-4120 extension 4751. Fax: 519-763-3117. E-mail: smcewen{at}ovcnet.uoguelph.ca.

dagger Present address: Agriculture Western Australia, Narrogin, Western Australia, 6312.




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Copyright © 1998 by the American Society for Microbiology. All rights reserved.