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Appl. Environ. Microbiol., Jul 1997, 2765-2770, Vol 63, No. 7
Copyright © 1997, American Society for Microbiology

Benzoate degradation via the ortho pathway in Alcaligenes eutrophus is perturbed by succinate

F Ampe, JL Uribelarrea, GM Aragao and ND Lindley
Centre de Bioingenierie Gilbert Durand, Institut National des Sciences Appliquees, Unite Mixte de Recherche CNRS 5044, Toulouse, France. ampe;lindley@insa-tlsc.fr

During batch growth of Alcaligenes eutrophus on benzoate-plus-succinate mixtures, substrates were simultaneously metabolized, leading to a higher specific growth rate (mu = 0.56 h-1) than when a single substrate was used (mu = 0.51 h-1 for benzoate alone and 0.44 h-1 for succinate alone), without adversely affecting the growth yield (0.57 Cmol/Cmol). Flux distribution analysis revealed that succinate dehydrogenase most probably controls the rate of total succinate consumption (the maximum flux being 9.7 mmol.g-1.h-1). It is postulated that the relative consumption rate of each substrate is in part related to modified levels of gene expression but to a large extent is dependent upon the presence of succinate, end product of the beta- ketoadipate pathway. Indeed, the in vitro beta-ketoadipate-succinyl coenzyme A transferase activity was seen to be inhibited by succinate, a coproduct of the reaction.

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