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Appl. Environ. Microbiol., Jul 1997, 2671-2678, Vol 63, No. 7
Copyright © 1997, American Society for Microbiology

Production and Identification of N-Glucosylrubropunctamine and N-Glucosylmonascorubramine from Monascus ruber and Occurrence of Electron Donor-Acceptor Complexes in These Red Pigments

H Hajjaj, A Klaebe, MO Loret, T Tzedakis, G Goma and PJ Blanc
Centre Bio-Ingenierie Gilbert Durand, CNRS UMR 5504, LA INRA, Institut National des Sciences Appliquees de Toulouse, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, and Laboratoire IMRCP, UMR-CNRS 5623, Groupe de Chimie Organique Biologique and Laboratoire de Genie Chimique et Electrochimie, UMR 5503, Universite Paul Sabatier, Toulouse Cedex, France

The filamentous fungus Monascus ruber produces water-soluble red pigments in a submerged culture when grown in a chemically defined medium containing glucose as a carbon source and monosodium glutamate as a nitrogen source. Two new molecules with polyketide structures, N-glucosylrubropunctamine and N-glucosylmonascorubramine, constituting under some conditions 10% of the total extracellular coloring matter when glucose as a carbon source was in excess (20 g/liter), were isolated and structurally characterized by high-pressure liquid chromatography, Dionex methods, (sup1)H and (sup13)C nuclear magnetic resonance spectroscopy, and mass spectrometry. The occurrence of the electron donor-acceptor complex effect was demonstrated by UV spectroscopy, polarography, and thin-layer voltammetry. The use of n-butanol as an extraction solvent stabilized the pigments against the effects of daylight for several months, promoting the stability of this type of complex.

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