This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, I. M. Articles by Davis, R. E. Search for Related Content PubMed PubMed Citation Articles by Lee, I. M. Articles by Davis, R. E. Agricola Articles by Lee, I. M. Articles by Davis, R. E.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Jul 1997, 2631-2636, Vol 63, No. 7
Copyright © 1997, American Society for Microbiology

Phylogeny and classification of bacteria in the genera Clavibacter and Rathayibacter on the basis of 16s rRNA gene sequence analyses

IM Lee, IM Bartoszyk, DE Gundersen-Rindal and RE Davis
Molecular Plant Pathology Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 20705, USA.

A phylogenetic analysis by parsimony of 16S rRNA gene sequences (16S rDNA) revealed that species and subspecies of Clavibacter and Rathayibacter form a discrete monophyletic clade, paraphyletic to Corynebacterium species. Within the Clavibacter-Rathayibacter clade, four major phylogenetic groups (subclades) with a total of 10 distinct taxa were recognized: (I) species C. michiganensis; (II) species C. xyli; (III) species R. iranicus and R. tritici; and (IV) species R. rathayi. The first three groups form a monophyletic cluster, paraphyletic to R. rathayi. On the basis of the phylogeny inferred, reclassification of members of Clavibacter-Rathayibacter group is proposed. A system for classification of taxa in Clavibacter and Rathayibacter was developed based on restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified 16S rDNA sequences. The groups delineated on the basis of RFLP patterns of 16S rDNA coincided well with the subclades delineated on the basis of phylogeny. In contrast to previous classification systems, which are based primarily on phenotypic properties and are laborious, the RFLP analyses allow for rapid differentiation among species and subspecies in the two genera.

This article has been cited by other articles:

• Bentley, S. D., Corton, C., Brown, S. E., Barron, A., Clark, L., Doggett, J., Harris, B., Ormond, D., Quail, M. A., May, G., Francis, D., Knudson, D., Parkhill, J., Ishimaru, C. A. (2008). Genome of the Actinomycete Plant Pathogen Clavibacter michiganensis subsp. sepedonicus Suggests Recent Niche Adaptation. J. Bacteriol. 190: 2150-2160 [Abstract] [Full Text]  
• Yin, P., Li, T.-Y., Xie, M.-H., Jiang, L., Zhang, Y. (2006). A Type Ib ParB Protein Involved in Plasmid Partitioning in a Gram-Positive Bacterium. J. Bacteriol. 188: 8103-8108 [Abstract] [Full Text]  
• Brown, S. E., Knudson, D. L., Ishimaru, C. A. (2002). Linear Plasmid in the Genome of Clavibacter michiganensis subsp. sepedonicus. J. Bacteriol. 184: 2841-2844 [Abstract] [Full Text]