This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stentz, R. Articles by Zagorec, M. Search for Related Content PubMed PubMed Citation Articles by Stentz, R. Articles by Zagorec, M. Agricola Articles by Stentz, R. Articles by Zagorec, M.

Next Article 

Appl. Environ. Microbiol., Jun 1997, 2111-2116, Vol 63, No. 6
Copyright © 1997, American Society for Microbiology

Molecular cloning and analysis of the ptsHI operon in Lactobacillus sake

R Stentz, R Lauret, SD Ehrlich, F Morel-Deville and M Zagorec
Laboratoire de Recherches sur la Viande, INRA, Jouy-en-Josas, France.

The ptsH and ptsI genes of Lactobacillus sake, encoding the general enzymes of the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS), were cloned and sequenced. HPr (88 amino acids), encoded by ptsH, and enzyme I (574 amino acids), encoded by ptsI, are homologous to the corresponding known enzymes of other bacteria. Nucleotide sequence and mRNA analysis showed that the two genes are cotranscribed in a large transcript encoding both HPr and enzyme I. The transcription of ptsHI was shown to be independent of the carbon source. Four ptsI mutants were constructed by single-crossover recombination. For all mutants, growth on PTS carbohydrates was abolished. Surprisingly, the growth rates of mutants on ribose and arabinose, two carbohydrates which are not transported by the PTS, were accelerated. This unexpected phenotype suggests that the PTS negatively controls ribose and arabinose utilization in L. sake by a mechanism different from the regulation involving HPr described for other gram- positive bacteria.

This article has been cited by other articles:

• Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
• Marceau, A., Zagorec, M., Chaillou, S., Mera, T., Champomier-Verges, M.-C. (2004). Evidence for Involvement of at Least Six Proteins in Adaptation of Lactobacillus sakei to Cold Temperatures and Addition of NaCl. Appl. Environ. Microbiol. 70: 7260-7268 [Abstract] [Full Text]  
• Champomier-Verges, M.-C., Marceau, A., Mera, T., Zagorec, M. (2002). The pepR Gene of Lactobacillus sakei Is Positively Regulated by Anaerobiosis at the Transcriptional Level. Appl. Environ. Microbiol. 68: 3873-3877 [Abstract] [Full Text]  
• Dudez, A.-M., Chaillou, S., Hissler, L., Stentz, R., Champomier-Verges, M.-C., Alpert, C.-A., Zagorec, M. (2002). Physical and genetic map of the Lactobacillus sakei 23K chromosome. Microbiology 148: 421-431 [Abstract] [Full Text]  
• Stentz, R., Loizel, C., Malleret, C., Zagorec, M. (2000). Development of Genetic Tools for Lactobacillus sakei: Disruption of the beta -Galactosidase Gene and Use of lacZ as a Reporter Gene To Study Regulation of the Putative Copper ATPase, AtkB. Appl. Environ. Microbiol. 66: 4272-4278 [Abstract] [Full Text]  
• Ingmer, H., Vogensen, F. K., Hammer, K., Kilstrup, M. (1999). Disruption and Analysis of the clpB, clpC, and clpE Genes in Lactococcus lactis: ClpE, a New Clp Family in Gram-Positive Bacteria. J. Bacteriol. 181: 2075-2083 [Abstract] [Full Text]