This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Huddleston, A. S. Articles by Wellington, E. M. Search for Related Content PubMed PubMed Citation Articles by Huddleston, A. S. Articles by Wellington, E. M. Agricola Articles by Huddleston, A. S. Articles by Wellington, E. M.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Apr 1997, 1288-1297, Vol 63, No. 4
Copyright © 1997, American Society for Microbiology

Molecular detection of streptomycin-producing streptomycetes in Brazilian soils

AS Huddleston, N Cresswell, MC Neves, JE Beringer, S Baumberg, DI Thomas and EM Wellington
Department of Biological Sciences, University of Warwick, Coventry, West Midlands, United Kingdom.

Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains were streptomycin resistance. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin- producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.

This article has been cited by other articles:

• Narisawa, N., Haruta, S., Arai, H., Ishii, M., Igarashi, Y. (2008). Coexistence of Antibiotic-Producing and Antibiotic-Sensitive Bacteria in Biofilms Is Mediated by Resistant Bacteria. Appl. Environ. Microbiol. 74: 3887-3894 [Abstract] [Full Text]  
• Groh, J. L., Luo, Q., Ballard, J. D., Krumholz, L. R. (2007). Genes That Enhance the Ecological Fitness of Shewanella oneidensis MR-1 in Sediments Reveal the Value of Antibiotic Resistance. Appl. Environ. Microbiol. 73: 492-498 [Abstract] [Full Text]  
• Davelos, A. L., Kinkel, L. L., Samac, D. A. (2004). Spatial Variation in Frequency and Intensity of Antibiotic Interactions among Streptomycetes from Prairie Soil. Appl. Environ. Microbiol. 70: 1051-1058 [Abstract] [Full Text]  
• Albuquerque de Barros, E. V. S., Manfio, G. P., Ribeiro Maitan, V., Mendes Bataus, L. A., Kim, S. B., Maldonado, L. A., Goodfellow, M. (2003). Nocardiacerradoensis sp. nov., a novel isolate from Cerrado soil in Brazil. Int. J. Syst. Evol. Microbiol. 53: 29-33 [Abstract] [Full Text]  
• Walker, J. B. (2002). Enzymatic Synthesis of Aminoglycoside Antibiotics: Novel Adenosylmethionine:2-Deoxystreptamine N-Methyltransferase Activities in Hygromycin B- and Spectinomycin-Producing Streptomyces spp. and Uses of the Methylated Products. Appl. Environ. Microbiol. 68: 2404-2410 [Abstract] [Full Text]  
• Ryding, N. J., Anderson, T. B., Champness, W. C. (2002). Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System. J. Bacteriol. 184: 794-805 [Abstract] [Full Text]  
• Egan, S., Wiener, P., Kallifidas, D., Wellington, E. M. H. (1998). Transfer of Streptomycin Biosynthesis Gene Clusters within Streptomycetes Isolated from Soil. Appl. Environ. Microbiol. 64: 5061-5063 [Abstract] [Full Text]