This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Thiery, I. Articles by Orduz, S. Search for Related Content PubMed PubMed Citation Articles by Thiery, I. Articles by Orduz, S. Agricola Articles by Thiery, I. Articles by Orduz, S.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 02 1997, 468-473, Vol 63, No. 2
Copyright © 1997, American Society for Microbiology

Identification of a gene for Cyt1A-like hemolysin from Bacillus thuringiensis subsp. medellin and expression in a crystal-negative B. thuringiensis strain

I Thiery, A Delecluse, MC Tamayo and S Orduz
Unite des Bacteries Entomopathogenes, Institut Pasteur, Paris, France. ithiery@pasteur.fr

A gene designated cyt1Ab1, encoding a 27,490-Da protein, was isolated from Bacillus thuringiensis subsp. medellin (H30 serotype) by using an oligonucleotide probe corresponding to the cyt1Aa1 gene. The sequence of the Cyt1Ab1 protein, as deduced from the sequence of the cyt1Ab1 gene, was 86% identical to that of the Cyt1Aa1 protein and 32% identical to that of the Cyt2Aa1 protein from B. thuringiensis subsp. kyushuensis. The cyt1Ab1 gene was flanked upstream by a p21 gene, in the same orientation, encoding a 21,370-Da protein that showed 84% similarity to the putative chaperone P20 protein from B. thuringiensis subsp. israelensis and downstream, on the opposite strand, by a sequence showing 85% identity to the IS240A insertion sequence. The cyt1Ab1 gene was expressed at a high level in a nontoxic strain of B. thuringiensis subsp. israelensis in which large inclusions of the Cyt1Ab1 protein were produced. Purified Cyt1Ab1 crystals were as hemolytic as those of the Cyt1Aa1 protein and were twice as hemolytic as those from the wild-type strain. Mosquitocidal activity toward Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae was assayed. The toxicity of the Cyt1Ab1 protein was slightly lower than that of the Cyt1Aa1 protein for all three mosquito species, and Cyt1Ab1 was 150, 300, and 800 times less active toward Culex, Anopheles, and Aedes larvae, respectively, than were the native crystals from B. thuringiensis subsp. medellin.

This article has been cited by other articles:

• Manasherob, R., Itsko, M., Sela-Baranes, N., Ben-Dov, E., Berry, C., Cohen, S., Zaritsky, A. (2006). Cyt1Ca from Bacillus thuringiensis subsp. israelensis: production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins.. Microbiology 152: 2651-2659 [Abstract] [Full Text]  
• Itsko, M., Manasherob, R., Zaritsky, A. (2005). Partial Restoration of Antibacterial Activity of the Protein Encoded by a Cryptic Open Reading Frame (cyt1Ca) from Bacillus thuringiensis subsp. israelensis by Site-Directed Mutagenesis. J. Bacteriol. 187: 6379-6385 [Abstract] [Full Text]  
• Manasherob, R., Zaritsky, A., Metzler, Y., Ben-Dov, E., Itsko, M., Fishov, I. (2003). Compaction of the Escherichia coli nucleoid caused by Cyt1Aa. Microbiology 149: 3553-3564 [Abstract] [Full Text]  
• Butko, P. (2003). Cytolytic Toxin Cyt1A and Its Mechanism of Membrane Damage: Data and Hypotheses. Appl. Environ. Microbiol. 69: 2415-2422 [Full Text]  
• Juarez-Perez, V., Guerchicoff, A., Rubinstein, C., Delecluse, A. (2002). Characterization of Cyt2Bc Toxin from Bacillus thuringiensis subsp. medellin. Appl. Environ. Microbiol. 68: 1228-1231 [Abstract] [Full Text]  
• Wirth, M. C., Delecluse, A., Walton, W. E. (2001). Cyt1Ab1 and Cyt2Ba1 from Bacillus thuringiensis subsp. medellin and B. thuringiensis subsp. israelensis Synergize Bacillus sphaericus against Aedes aegypti and Resistant Culex quinquefasciatus (Diptera: Culicidae). Appl. Environ. Microbiol. 67: 3280-3284 [Abstract] [Full Text]  
• Guerchicoff, A., Delécluse, A., Rubinstein, C. P. (2001). The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family. Appl. Environ. Microbiol. 67: 1090-1096 [Abstract] [Full Text]  
• Wirth, M. C., Federici, B. A., Walton, W. E. (2000). Cyt1A from Bacillus thuringiensis Synergizes Activity of Bacillus sphaericus against Aedes aegypti (Diptera: Culicidae). Appl. Environ. Microbiol. 66: 1093-1097 [Abstract] [Full Text]  
• Thiéry, I., Hamon, S., Delécluse, A., Orduz, S. (1998). The Introduction into Bacillus sphaericus of the Bacillus thuringiensis subsp. medellin cyt1Ab1 Gene Results in Higher Susceptibility of Resistant Mosquito Larva Populations to B. sphaericus. Appl. Environ. Microbiol. 64: 3910-3916 [Abstract] [Full Text]  
• Crickmore, N., Zeigler, D. R., Feitelson, J., Schnepf, E., Van Rie, J., Lereclus, D., Baum, J., Dean, D. H. (1998). Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 807-813 [Abstract] [Full Text]