This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fraaije, M. W. Articles by Van Berkel, W. Search for Related Content PubMed PubMed Citation Articles by Fraaije, M. W. Articles by Van Berkel, W. Agricola Articles by Fraaije, M. W. Articles by Van Berkel, W.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Feb 1997, 435-439, Vol 63, No. 2
Copyright © 1997, American Society for Microbiology

Enigmatic Gratuitous Induction of the Covalent Flavoprotein Vanillyl-Alcohol Oxidase in Penicillium simplicissimum

MW Fraaije, M Pikkemaat and WJH Van Berkel
Department of Biochemistry, Agricultural University, 6703 HA Wageningen, The Netherlands

When Penicillium simplicissimum is grown on veratryl alcohol, anisyl alcohol, or 4-(methoxymethyl)phenol, an intracellular covalent flavin-containing vanillyl-alcohol oxidase is induced. The induction is highest (up to 5% of total protein) during the growth phase. In addition to vanillyl-alcohol oxidase, an intracellular catalase-peroxidase is induced. Induction of vanillyl-alcohol oxidase in P. simplicissimum is prevented by the addition of isoeugenol to veratryl alcohol-containing media, but growth is unaffected. The inhibitory effect of isoeugenol on induction is not observed when anisyl alcohol or 4-(methoxymethyl)phenol is used as the growth substrate. Based on the induction experiments and the degradation pathways for veratryl and anisyl alcohol, we propose that induction of vanillyl-alcohol oxidase is superfluous when P. simplicissimum is grown on these aromatic alcohols. However, the enzyme plays an essential role in the degradation of the methyl ether of p-cresol, 4-(methoxymethyl)phenol.

This article has been cited by other articles:

• Winkler, A., Kutchan, T. M., Macheroux, P. (2007). 6-S-Cysteinylation of Bi-covalently Attached FAD in Berberine Bridge Enzyme Tunes the Redox Potential for Optimal Activity. J. Biol. Chem. 282: 24437-24443 [Abstract] [Full Text]  
• Heuts, D. P. H. M., van Hellemond, E. W., Janssen, D. B., Fraaije, M. W. (2007). Discovery, Characterization, and Kinetic Analysis of an Alditol Oxidase from Streptomyces coelicolor. J. Biol. Chem. 282: 20283-20291 [Abstract] [Full Text]  
• Tahallah, N., van den Heuvel, R. H. H., van den Berg, W. A. M., Maier, C. S., van Berkel, W. J. H., Heck, A. J. R. (2002). Cofactor-dependent Assembly of the Flavoenzyme Vanillyl-alcohol Oxidase. J. Biol. Chem. 277: 36425-36432 [Abstract] [Full Text]  
• Shimoni, E., Baasov, T., Ravid, U., Shoham, Y. (2002). The trans-Anethole Degradation Pathway in an Arthrobacter sp.. J. Biol. Chem. 277: 11866-11872 [Abstract] [Full Text]  
• van den Heuvel, R. H. H., Fraaije, M. W., Mattevi, A., van Berkel, W. J. H. (2000). Asp-170 Is Crucial for the Redox Properties of Vanillyl-alcohol Oxidase. J. Biol. Chem. 275: 14799-14808 [Abstract] [Full Text]  
• Fraaije, M. W., van den Heuvel, R. H. H., van Berkel, W. J. H., Mattevi, A. (1999). Covalent Flavinylation Is Essential for Efficient Redox Catalysis in Vanillyl-alcohol Oxidase. J. Biol. Chem. 274: 35514-35520 [Abstract] [Full Text]  
• van den Heuvel, R. H. H., Fraaije, M. W., Laane, C., van Berkel, W. J. H. (1998). Regio- and Stereospecific Conversion of 4-Alkylphenols by the Covalent Flavoprotein Vanillyl-Alcohol Oxidase. J. Bacteriol. 180: 5646-5651 [Abstract] [Full Text]  
• Benen, J. A. E., Sanchez-Torres, P., Wagemaker, M. J. M., Fraaije, M. W., van Berkel, W. J. H., Visser, J. (1998). Molecular Cloning, Sequencing, and Heterologous Expression of the vaoA Gene from Penicillium simplicissimum CBS 170.90 Encoding Vanillyl-Alcohol Oxidase. J. Biol. Chem. 273: 7865-7872 [Abstract] [Full Text]  
• Fraaije, M. W., van Berkel, W. J. H. (1997). Catalytic Mechanism of the Oxidative Demethylation of 4-(Methoxymethyl)phenol by Vanillyl-Alcohol Oxidase. EVIDENCE FOR FORMATION OF A p-QUINONE METHIDE INTERMEDIATE. J. Biol. Chem. 272: 18111-18116 [Abstract] [Full Text]  
• van den Heuvel, R. H. H., Fraaije, M. W., Ferrer, M., Mattevi, A., van Berkel, W. J. H. (2000). Inversion of stereospecificity of vanillyl-alcohol oxidase. Proc. Natl. Acad. Sci. USA 97: 9455-9460 [Abstract] [Full Text]