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Appl. Environ. Microbiol., 10 1996, 3614-3619, Vol 62, No. 10
Copyright © 1996, American Society for Microbiology

Malt-yeast extract-sucrose agar, a suitable medium for enumeration and isolation of fungi from silage

I Skaar and H Stenwig
Department of Bacteriology and Feed Hygiene, Central Veterinary Laboratory, Oslo, Norway. Ida.Skaar@vetinst.no

A general medium named malt-yeast extract-sucrose agar (MYSA) containing oxgall was designed. The medium was intended for the enumeration and isolation of molds and yeasts in routine examinations of animal feed stuffs. In this study MYSA was tested as a general medium for mycological examination of silage. The medium was compared with dichloran-rose bengal medium (DRBC) in an examination of more than 500 specimens of big bale grass silage. Selected characteristics of known fungal species commonly isolated from feeds were examined after growth on MYSA and DRBC and on malt extract agar, used as a noninhibitory control medium. MYSA suppressed bacterial growth, without affecting the growth of fungi common in feeds. The fungi growing on MYSA were easily recognized, and the medium seemed to slow radial growth of fungal colonies, which permitted, easy counting. The number of species found was higher on MYSA than on DRBC. When we compared MYSA with DRBC for mycological examination of grass silage samples, MYSA was found to be the medium of choice.


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Copyright © 1996 by the American Society for Microbiology. All rights reserved.