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Appl. Environ. Microbiol., Jan 1996, 184-190, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Investigation of the Sterol Composition and Azole Resistance in Field Isolates of Septoria tritici

T Joseph-Horne, D Hollomon, N Manning and SL Kelly
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, and Neonatal Screening Laboratory, Sheffield Childrens Hospital, Western Bank, Sheffield S10 2UH, and IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS18 9AF, United Kingdom

We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL2 and S-27 contained isomers of ergosterol and ergosta-5,7-dienol, although in differing concentrations. Under azole treatment, this phytopathogen exhibited a response similar to that of other pathogenic fungi, with a reduction in desmethyl sterols and an accumulation of 14(alpha)-methyl sterols, indicative of inhibition of the P450-mediating sterol 14(alpha)-demethylase. Growth arrest was attributed to the reduction of ergosterol combined with an accumulation of nonutilizable sterols. Strain S-27 exhibited an azole-resistant phenotype which was correlated with decreased cellular content of azole.

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