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Appl. Environ. Microbiol., Jan 1996, 152-155, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Production of R-(-)-Ketoprofen from an Amide Compound by Comamonas acidovorans KPO-2771-4

K Yamamoto, K Otsubo, A Matsuo, T Hayashi, I Fujimatsu and K Komatsu
Technology Development Department, Pharmaceutical Production & Technology Center, Asahi Chemical Industry Co. Ltd., 632-1 Mifuku, Ohito-cho, Tagata-gun, Shizuoka 410-23, Japan

R-(-)-2-(3(prm1)-Benzoylphenyl)propionic acid [R-(-)-ketoprofen] was produced from racemic 2-(3(prm1)-benzoylphenyl)propionamide (keto-amide) by the isolated bacterial strain Comamonas acidovorans KPO-2771-4. Sodium fumarate as the carbon source and 2-azacyclononanone or isobutyronitrile as the enhancer in the culture medium were effective for bacterial growth and the enhancement of R-(-)-ketoprofen-producing activity. R-(-)-Ketoprofen produced from the keto-amide by resting cells was present in 99% enantiomeric exess. C. acidovorans KPO-2771-4 has an R-enantioselective amidase for keto-amide because the purified amidase from the bacterium hydrolyzed keto-amide, producing optically pure R-ketoprofen and ammonia.