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Appl. Environ. Microbiol., 09 1995, 3221-3226, Vol 61, No. 9
Copyright © 1995, American Society for Microbiology

Phylogenetic comparison of two polycyclic aromatic hydrocarbon- degrading mycobacteria

M Govindaswami, DJ Feldhake, BK Kinkle, DP Mindell and JC Loper
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524, USA.

Two mycobacterial strains previously isolated from fossil-fuel- contaminated environments and shown to degrade four- and/or five-ring polycyclic aromatic hydrocarbons were further characterized. The two strains, PYR-I and RJGII-135, had similar growth characteristics, colony morphologies, and scotochromogenic pigmentations. DNA amplification fingerprints obtained with total genomic DNA indicated some strain similarities but with several distinctly different bands. Moreover, phylogenetic analysis based upon essentially full-length 16S rRNA gene sequences separates the two strains as distinct species within the fast-growing group of mycobacteria. Although both strains are thermosensitive, strain PYR-I has the bulged U between positions 184 and 193 characteristic of thermotolerant mycobacteria. Both strains are of potential use for reintroduction into and bioremediation of polycyclic aromatic hydrocarbon-contaminated soils.

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