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Appl. Environ. Microbiol., Aug 1995, 2981-2989, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Use of Controlled Luciferase Expression To Monitor Chemicals Affecting Protein Synthesis

J Lampinen, M Virta and M Karp
Department of Biochemistry and Pharmacy, Abo Akademi University, FIN-20521 Abo, Finland, and Department of Biochemistry and Biotechnology, University of Turku, FIN-20520 Turku, Finland

In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene from Pyrophorus plagiophthalamus was found to be the best of three types of luciferase genes tested. Chemicals with known inhibitory effects on protein synthesis were used as test chemicals together with some general toxicants. The incubation of a test chemical with cells was performed either prior to or after the induction of protein synthesis, and the difference in the results of the two methods distinguishes the possible influence on protein synthesis from direct metabolic inhibition. Using lyophilized bacteria, the test is performed in less than an hour without any bacterial cultivation, which makes the test suitable for rapid and sensitive screening of chemicals or environmental samples. Compared with the standardized 50% inhibitory concentration calculation method of the bioluminescent cytotoxicity test, the more direct approach of calculation developed in this study proved to be more convenient than and as reliable as the standard method.

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